反相高效液相色谱法测定黄芪注射液中黄芪甲苷含量

摘要

目的：测定黄芪注射液中黄芪甲苷的含量。方法：采用RP-HPLC法，流动相为乙腈-水-磷酸（1∶2∶0.1），检测波长为203nm。结果：线性方程为Y=7.89×104X+1.16×104，r=0.9992，黄芪甲苷在1.26～12.6μg范围内线性关系良好，平均回收率为94.7%，RSD%为2.6%(n＝6)。结论：建立的含量测定方法简便、准确。%OBJECTIVE To develop a water for determination of astragoloside Ⅳin the astragale injection.METHODS Using RP-HPLC,the mobile phase consisted acetonitrile-water-phosphoric acid (1∶2∶0.1), the detection wavelength was 203 nm.RESULTS The regression equation was Y=7.89×104X+1.16×104, r＝0.999 2, (1.26～12.6 μg). The average recovery was 94.71%, RSD=2.6%(n＝6).CONCLUSIONS The method was simple and accurate.