恶性胸腔积液中肺腺癌的EGFR、VEGF和P53的表达及分子生物学意义

摘要

目的：探讨在恶性胸腔积液中肺腺癌细胞中 EGFR、VEGF、P53表达的状态和关系及对临床治疗的分子生物学意义。方法利用液基薄层细胞学自动涂片技术方法和免疫化学方法筛查出恶性胸腔积液中肺腺癌77例和良性胸水10例,在用免疫细胞化学染色检测 EGFR、VEGF、P53的表达情况。结果免疫细胞化学染色显示 EGFR 在肿瘤细胞中阳性表达55例,表达率为71.4%；VEGF和 P53在恶性肿瘤细胞中阳性表达分别为57和31例,表达率分别为74%和40.3%,EGFR与 VEGF表达具有相关性(r=0.609；P=0.000)。结论 EGFR、VEGF和 P53在恶性胸腔积液的肺腺癌细胞中具有较高的表达率,且表达具有紧密联系,并可以对临床治疗有一定参考性。%Obj ective To investigate the expressions of EGFR,VEGF,P5 3 their is molecular biolog-ical significance in clinical treatment of malignant pleural effusion of lung adenocarcinoma cells.Methods ThinPrep cytology smear technical methods and automated immunochemical methods screened 77 cases of malignant pleural effusion of lung adenocarcinoma and 10 cases of benign pleural effusion.The expressions of EGFR,VEGF,P5 3 were detected by immunocytochemistry.Results In tumor cells,immunocytochem-istry showed EGFR expression in 5 5 cases and the expression rate was 7 1 .4%.VEGF and P5 3 were ex-pressed in 57 and 31 cases,respectively,and the expression rates were 74% and 40.3%.EGFR was corre-lated with VEGF (r=0.609;P=0.000).Conclusion EGFR,VEGF and P53 are highly expressed in ma-lignant pleural effusion of lung adenocarcinoma cells,and their expressions are closely correlated,and may have reference value for clinical treatment.