Objective To explore the roles of IL-6, IL-8 and TNFa in pathogenesis of acute hemorrhagic necrotizing pancreatitis (AHNP) and investigate the curative effects of different abdominal lavage fluids on AHNP. Methods A total of 160 rats were randomized into the sham operated group, control group, antibiotic abdominal lavaged group and traditional Chinese medicine (TCM) abdominal lavaged group. The model of AHNP was established by retrograde injection of 5% sodium taurocholate solution into the biliopancreatic duct. Then pancreatic tissue and blood samples were collected at the 24th, 48th and 72nd h after AHNP to measure plasma levels of IL-6, IL-8 and TNF-a and examine pancreatic histological changes. Results Plasma levels of IL-6, IL-8 and TNF-a were significantly higher in the control group than in the sham operated group. Meanwhile, they were increased with aggravation of AHNP. In the lavaged groups, pathological changes of the pancreas were ameliorated. TCM was more effective than other antibiotics in protecting the pancreas and reducing plasma levels of IL-6, IL-8 and TNF-a. Conclusions IL-6, IL-8 and TNF-a might be involved in pathogenesis of AHNP. Thus detection of plasma levels of them is helpful for evaluating the severity of AHNP. TCM abdominal lavage is more effective method for reducing plasma levels of IL-6, IL-8 and TNFa and alleviating pancreatic damage. Meanwhile, it is simple, safe and effective.%目的 探讨白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子α（TNF-α）在急性出血坏死性胰腺炎（AHNP）发病中的作用，观察各种腹腔灌洗液对AHNP的治疗效果。方法 将160只大鼠随机分成：假手术组、对照组、抗生素灌洗组和中药灌洗组。采用5％牛磺胆酸钠逆行胆胰管注射建立大鼠AHNP模型，并于模型制成后24 h、48 h、72 h取血液、腹水、胰腺组织，测定血液、腹水中IL-6、IL-8、TNF-α水平，作胰腺病理检查。结果 对照组大鼠血液、腹水中IL-6、IL-8、TNF-α水平较假手术组明显增高，并随病情加重而升高，胰腺组织学改变进行性加重。经腹腔灌洗治疗后各组上述指标水平有所降低，胰腺病理损害减轻，以中药腹腔灌洗组改善最为明显。结论 IL-6、IL-8、TNF-α参与AHNP发病机理，检测上述指标有助于对AHNP病情轻重程度的评估。中药腹腔灌洗能够更好地降低血液、腹水中IL-6、IL-8、TNF-α水平，减轻胰腺组织的损害。中药腹腔灌洗治疗AHNP方法简单、安全、有效。
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