目的 探讨胰腺癌细胞Panc-1中AKT2基因表达对吉西他滨敏感性的影响.方法 体外将AKT2特异性shRNA表达载体pAKT2-shRNA转染胰癌细胞株Panc-1.应用RT-PCR、Western blot检测转染shRNA后Panc-1细胞AKT2基因和蛋白的表达变化,应用MTT法检测干扰AKT2后Panc-1细胞对吉西他滨敏感性的变化.结果 转染pAKT2-shRNA后Panc-1细胞AKT2基因和蛋白的表达水平明显下降;吉西他滨对Panc-1细胞的半数抑制量从(1.96±0.22) mg/L降到(0.24±0.03)mg/L,Panc-1细胞对吉西他滨的敏感性明显增加.结论 用pAKT2-shRNA抑制AKT2基因表达,能增加人胰癌细胞株Panc-1对吉西他滨的敏感性.%Objective To explore the effects of AKT2 expression in pancreatic cancer cell line Panc-1 on the sensitivity towards to gemcitabine.Methods The expression vector pAKT2 shRNA was constructed and transfected into Panc-1 cells by lipofecton.The mRNA and protein expression levels of AKT2 were detected by RT-PCR and Western blot assays,respectively.The changes of gemcitabine sensitivity after shRNA were examined by the MTT assay.Results The mRNA and protein levels of AKT2 in Panc-1 cells were significantly decreased after the transfection,and the median inhibition concentration of gemcitabine against Panc-1 cells was reduced from (1.96 ± 0.22) mg/L to (0.24±0.03) mg/L.The sensitivity of Panc-1 cells to gemcitabine was increased significantly after pAKT2-shRNA transfection.Conclusion The sensitivity of Panc-1 cells to gemcitabine could be enhanced by pAKT2-shRNA.
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