氟伐他汀防治大鼠放射性胸膜炎的实验研究

摘要

目的：观察氟伐他汀对大鼠放射性胸膜炎的防治作用。方法采用随机数字表法，将80只健康雌性SD大鼠分为对照组（n=8）、单纯放射组（n=36）、氟伐他汀组（n=36）。氟伐他汀组于照射前1周，给予20mg/（kg•d）氟伐他汀灌胃，直至活杀，其他组灌服等量生理盐水。单纯放射组和氟伐他汀组采用直线加速器全胸部照射，单次剂量20Gy。照射后对大鼠进行X线透视扫描，并在第15d、30d、60d以3.5%水合氯醛麻醉大鼠，剪开胸腔，对有胸水者进行病理和生化检查。结果单纯放射组放射性胸膜炎发生率为41.7%（15/36）。氟伐他汀组放射性胸膜炎发生率为19.4%（7/36）。两组之间的差异有统计学意义（χ2=4.19，P＜0.05）。经过对比发现氟伐他汀组病理学改变轻于同期的单纯放射组。结论放射性胸膜炎在放射性肺损伤的早期即可出现，氟伐他汀能有效防治大鼠放射性胸膜炎。%Objective To study the protective effect of fluvastatin on radiation-induced pleurisy model in rats.Methods 80 Healthy SD rats(180±20g) were randomly divided into 3 groups:normal control(n=8),radiation control(n=36) and radiation plus treatment with fluvastatin groups(n=36).The dose of thoracic radiation was 20 Gy by linear accelerator in 72 rats in the latter two groups after anesthesia.7 days prior to radiation,fluvastatin was given to the treatment group at a dose of 20 mg/kg per day by gastrogavage until the end of the experiment. The animals in the other groups were given same amount of saline by gastrogavage.The rats were scanned by X-ray after radiation.Chloral hydrate was injected intraperitoneally at a dose of 3.5%on 15, 30 and 60 days after radiation.After they were anesthetized,their thoraces were opened.If there was pleurorrhea,routine and biochemical examinations were carried out.Hematoxylin and eosin(H&E) stain were performed on the lung tissue slices to observe changes in microstructure.The data were analyzed by univariate of general linear model test of SPSS 13.0.Results Pleurisy induced by radiation was found in 22 of 72 rats.Incidence rate in radiation control group was 41.7%(15/36) and that in radiation plus treatment group was 19.4%(7/36).Compared to radiation control group,the pathologic change alleviated,and the rate of pleurisy in radiation plus treatment group decreased signiifcantly(P<0.05).Conclusion Pleurisy can occur in the early stage of radiation-induced lung injury.Fluvastatin has certain protective effect on radiation-induced pleurisy.