益气养阴消癥通络中药对糖尿病大鼠肾脏纤维化的保护作用研究

摘要

目的 探讨益气养阴消癥通络中药对糖尿病大鼠肾脏的保护作用及其抗纤维化作用的可能机制.方法 将SD大鼠随机分为6组；单纯肾切组、模型组、厄贝沙坦组、中药低、中、高剂量组,按各组相应药物剂量灌胃.于给药第6周末检测各组大鼠24h尿蛋白(U Pro)量；光镜下观察各组大鼠肾脏的病理改变；采用RT-PCR方法检测各组大鼠肾皮质Ⅳ型胶原的mRNA表达水平,Western印迹方法检测各组大鼠肾皮质结缔组织生长因子(CTGF)的蛋白表达水平.结果 与模型组比较,厄贝沙坦、中药低、中剂量组U Pro量显著下降(P＜0.05)；厄贝沙坦、中药低剂量组Col-ⅣmRNA和CTGF蛋白表达均减少(P＜0.05)；各治疗组与模型组比较,病理改变有所减轻.结论 抑制CTGF/Col-Ⅳ的过度表达可能是益气养阴消癥通络中药抗糖尿病大鼠肾脏纤维化作用的机制之一.%Objective To investigate the anti-fibrosis effects and molecular biology mechanism of supplementing qi-nourishing yin and disperse blood stasis-dredge collateral herbs on the kidney of the diabetic rats. Methods SD rats were randomly divided into 6 groups: simple nephrectomy,model,irbesatan,low,middle,and high dose TCM groups,and afforded with the medicine respectively. At the end of the sixth week,24 h urine protein (U Pro) , pathomorphology observations and the Col-IV mRNA and CTGF protein expressions were detected. Results Compared with that of model,irbesatan,TCM low dose and middle dose groups the levels of U Pro were significantly decreased (P < 0. 05). Levels of Col-IV mRNA and CTGF protein expressions were significantly decreased in irbesatan and TCM low dose groups (P < 0.05). In all treated groups the pathological changes were also improved (P < 0. 05). Conclusions Supplementing qi-nourishing yin and disperse blood stasis-dredge collateral herbs could inhibit the fibrosis in the kidney of diabetic rats effectively by down regulating the expression of CTGF/Col-IV.