广西巴马长寿人群ACE基因多态性与环境因素的交互作用

摘要

目的 探讨血管紧张素转换酶(ACE)基因I/D多态性与环境因素在巴马地区长寿现象发生中的交互作用.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对广西巴马地区200例长寿老人(年龄90～110岁,长寿组)以及288例健康成年人(年龄48～89岁,对照组)的ACE基因进行基因分型.应用条件Logistic回归模型分析巴马长寿发生的环境及遗传因素,根据交互作用指标判断基因与环境的交互作用.结果 长寿区的环境因素及ACE基因的ID+DD基因型是巴马人长寿的促进因素,两者存在交互作用(OR=11.063,r=1.378).结论 ACE基因I/D多态性与环境因素在巴马地区长寿现象发生中存在一定的交互作用,其长寿现象的发生是遗传及环境因素共同作用的结果.%Objective To investigate the interaction of the environmental factors of the longevity district and ACE gene polymorphisms in Bama longevity. Methods Genotyping of ACE gene was performed by PCR-RFLP for 200 Longevous people ( aged 90 ～ 110 ) and 288 healthy controls ( aged 48 ～ 89). All data were performed with statistics and logistic regression analysis by SPSS. Results The interaction and confounding among the factors were estimated, and there was a positive interaction between the ID + DD genotypes in ACE and the environment of the longevity district. Conclusions The environment of the longevity district would be closely correlated with human longevity in Bama county. The polymorphisms of ACE gene with the environment factor have a synergistic effect on longevity. The longevity of Bama may be the interaction of heredity and environment.