细胞周期抑制因子p16可诱导人成纤维细胞发生衰老样变化

摘要

Objective　To investigate the involvement of cell cycle negative regulator p16 in the replicative senescence of normal human diploid fibroblasts. 　Methods　 p16 cDNA and retroviral vector was introduced into normal human fibroblast 2BS cells by transfection technique. Then the effects of p16 on replicative cellular senescence of 2BS cells were examined. 　Results　 Compared with the control cells, 2BS cells transfected with p16 cDNA showed significant suppression of growth rate (decrease 50%) with cell cycle arrested at G1 phase. This phenomenon similar to that of the senescent cells, with the cellular response to stimulation by growth factor decreased 79.4%, showing the characteristics in morphology of senescent fibroblasts. 　Conclusions　 The over expression of p16 gene contributes to the process of cellular senescence of normal human diploid fibroblasts.%目的探讨细胞周期抑制因子p16在细胞衰老中的作用。方法利用逆转录病毒载体将p16基因转染入人胚肺二倍体成纤维细胞2BS中,获得高表达，检测其对2BS细胞衰老的影响。结果与对照组细胞相比,p16基因转染后,2BS细胞生长速率下降了约50%,细胞周期阻滞于G1期,细胞对生长因子剌激的反应性下降了79.4%,细胞形态呈衰老细胞样变化。结论 p16在人二倍体成纤维细胞的衰老过程中起促进作用。