肌萎缩侧索硬化患者体内氧化压力的定量分析

摘要

Objective To measure the urinary 8-isoprostaglandin F2a (8-isoPGF2a)and in vitro oxidized low density lipoprotein ( ox-LDL) lag time in patients with amyotrophic lateral sclerosis ( ALS). Methods Nineteen ALS patients served as an ALS group according to the EL Escorial diagnosis criteria, and 19 age- and influence factors matched healthy subjects served as a control group in this study. Their in vitro ox-LDL lag time and urinary 8-isoPGF2a level were measured by copper oxidation-conjugated diene assay and ELISA, respectively. Results No significant difference was found in urinary 8-isoPGF2a level between ALS group and control group although it was higher in ALS group than in control group (48. 28 ± 4. 78 vs 47. 25 ± 2. 32 ng/mmol creatinine, P >0. 05). The in vitro ox-LDL lag time was shorter in ALS group than in control group (59. 32 ± 8. 65 vs 83. 16 ± 8. 37 min,P<0. 01). Conclusion Elevated LDL oxidative stress and reduced 8-isoPGF2a antioxidant ability play a certain role in pathogenesis of ALS, suggesting that effective antioxidant therapy is of certain clinical significance in controlling progression of ALS and improving its prognosis.%目的 检测肌萎缩侧索硬化(ALS)患者尿液中8-异前列腺素F2a(8-iso-PGF2a)浓度和LDL体外氧化延迟时间的变化,评价ALS患者体内的氧化压力及抗氧化能力情况.方法 根据EL Escorial诊断标准选择ALS患者19例为ALS组,同时选择年龄、影响因素相匹配的健康成年男性19例为对照组.通过铜氧化共轭双烯法检测LDL体外氧化延迟时间,酶联免疫吸附法检测尿液中8-iso-PGF2a浓度.结果 与对照组比较,ALS组尿液中8-iso-PGF2a浓度虽有升高趋势[(48.28±4.78) ng/mmol肌酐vs(47.25±2.32)ng/mmol肌酐],但差异无统计学意义(P＞0.05),而LDL体外氧化延迟时间明显缩短[(59.32±8.65)min vs (83.16±8.37)min],差异有统计学意义(P＜0.01).结论 增高的氧化压力与降低的抗氧化能力在ALS的发病机制中具有一定作用.提示有效的抗氧化治疗可能对控制ALS的发展及改善预后有一定的临床意义.