miR-29a调控HDAC4促肝癌细胞增殖和转移的机制研究

摘要

目的 检测miR-29a在肝癌中的表达,探讨其对肝癌细胞增殖及细胞周期的影响.方法 收集40例肝癌及癌旁组织标本,实时荧光定量PCR法检测miR-29a表达;选取肝癌细胞株HepG2,经转染miR-29a mimic后,采用CCK-8法检测增殖曲线,流式细胞仪检测细胞周期及Western blotting实验分析其可能作用机制.结果 与癌旁组织相比,肝癌组织中miR-29a表达明显下调(P＜0.05);在肝癌细胞株HepG2中上调miR-29a表达后可抑制肿瘤细胞增殖,引起G,期阻滞,上调HDAC4表达后这一现象可逆转.结论 肝癌中miR-29a显著低表达,并可能通过靶向调控HDAC4表达导致肝癌细胞增殖异常和周期阻滞.%Objective To detect the expression of miR-29a in the hepatocellular carcinoma (HCC) tissues,and to investigate the effect of miR-29a regulating the proliferation and metastasis of HCC cells.Methods Real time fluorescence quantitative PCR method was used to detect the expression of miR-29a in 40 cases of HCC tissue and adjacent tissue.The proliferation curve was detected by CCK-8 method after transfection with mimic miR-29a,and the cell cycle and Western blotting ting assay were used to detect the cell cycle and the possible mechanism.Results The expressi on of miR-29a was lower in HCC than that in the adjacent tissue (P ＜ 0.05).In HCC cell line HepG2,overexpression of miR-29a could inhibited tumor cell proliferation,induced G1 arrest.But,overexpression of HDAC4 could reversed this phenomenon.Conclusion miR-29a is lower expression in HCC significantly,and may lead to abnormal proliferation and cycle arrest of HCC cells by targeting regulation of HDAC4 expression.