目的 分析第10号染色体缺失的磷酸酶及张力蛋白(phosphatase and tensin homolog deleted on chro-mosome ten,PTEN)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)、细胞周期蛋白依赖性激酶抑制蛋白27(cyclin dependent kinase inhibitor protein 27,p27kip1)在乳腺癌组织中的表达情况,讨论其在乳腺癌发生、发展中的意义.方法 选取2014年2月至2016年2月本院肿瘤科和乳腺外科收治的55例乳腺癌患者(乳腺癌组)和35例乳腺良性病变患者(乳腺良性病变组)为研究对象.采用免疫组织化学SP法检测入选患者PTEN、MMP-9、p27kip1的表达水平.结果 乳腺良性病变组PTEN和p27kip1的表达率(82.86%,85.71%)均显著高于乳腺癌组(45.45%,43.64%)(P<0.05),MMP-9的表达率(42.86%)显著低于乳腺癌组(69.09%)(P<0.05).乳腺癌组织中PTEN的表达与MMP-9的表达呈负相关(r=-0.587,P<0.05),与p27kip1的表达呈正相关(r=0.532,P<0.05).结论 PTEN与p27kip1表达异常可能与乳腺癌的发生、发展及预后相关.MMP-9参与乳腺癌的发生、发展,可作为评估乳腺癌浸润和转移的生物学指标.%Objective To analyze the expression of phosphatase and tensin homolog deleted on chro-mosome ten (PTEN), matrix metalloproteinase-9 (MMP-9) and cyclin dependent kinase inhibitor protein 27 (p27kip1) and to discuss the significance of breast cancer. Method 55 patients with breast cancer (breast cancer group) and 35 patients with benign breast lesions (benign breast lesions group) from February 2014 to February 2016 in our hospital were selected as subjects investigated. The expression levels of PTEN, MMP-9 and p27kip1 were detected by immunohistochemical SP method. Result The expression rates of PTEN and p27kip1 of breast benign lesions group (82.86%, 85.71%) were significantly higher than that of breast cancer group (45.45%, 43.64%) (P < 0.05), the expression rate of MMP-9 (42.86%) was significantly lower than that of breast cancer group (69.09%) (P < 0.05). MMP-9 had negative correlation with PTEN in breast cancer (r = -0.587, P < 0.05), and was positively correlated with p27kip1 expression (r = 0.532, P < 0.05). Conclusion The abnormal expression of PTEN and p27kip1 may be related to the occurrence, development and prognosis of breast cancer. MMP-9 is involved in the development and progression of breast cancer, and can be used as a biological marker for evaluating the invasion and metastasis of breast cancer.
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