子宫内膜异位症异位和在位内膜中水通道蛋白1的表达及意义

摘要

目的:研究子宫内膜异位症患者子宫内膜中水通道蛋白1( AQP1)、血管内皮生长因子(VEGF)的表达及其相关性,探讨子宫内膜异位症的发病机制.方法:采用链霉素抗生物素蛋白-过氧化物酶连接法对36例子宫内膜异位症患者异位内膜及在位内膜,22例正常妇女(对照组)子宫内膜进行AQP1、VEGF的表达检测并进行相关性分析.结果:AQP1主要在微血管内皮细胞质中表达,VEGF主要在微血管内皮及间质细胞质中表达.异位内膜AQP1、VEGF表达强度的半定量免疫组化评分( HSCORE)值均高于在位内膜(P＜0.05)；在位内膜AQP1、VEGF表达强度的HSCORE值均高于对照组内膜(P＜0.05)；增殖期异位内膜AQP1、VEGF表达强度的HSCORE值均明显高于分泌期(P＜0.01)；增殖期在位内膜和对照组内膜AQP1、VEGF表达强度的HSCORE值与分泌期比较,差异均无显著性；AQP1、VEGF在子宫内膜异位症组异位内膜、在位内膜的表达均有显著正相关性(r=0.774,P＜0.01；r=0.749,P＜0.01)；AQP1和VEGF在对照组内膜的表达则均无相关性.结论:AQP1、VEGF在异位内膜细胞高表达,可能推动异位内膜黏附和侵袭,AQP1与VEGF可能通过促血管生成,共同促进异位内膜种植与生长,在子宫内膜异位症发生发展中起重要作用.%Objective: To study expressions and correlations of aquaporin - 1 ( AQP1 ) and vascular endothelial growth factor ( VEGF) in samples of endometrium from women suffering from endometriosis and to explore the pathogenesis of endometrio-sis. Methods; Expressions of AQP1 and VEGF were evaluated by immunohistochemical staining in ectopic and eutopic endom-etria from 36 women suffering from endometriosis (the study group) and 22 samples of normal endometrium were as the control. The scoring system ( HSCORE) that incorporated both the intensity and the distribution of specific staining and was used. Spearmans rank correlation were conducted for statistical analysis. Results; AQP1 was mainly expressed in endothelial cells of capillaries, while VEGF was in endothelial cells of capillaries and interstitial cells. Expression levels of AQP1 and VEGF in the ectopic endometrium were higher than those of the eutopic endometrium in the study group ( P < 0.05). And expression levels of AQP1 and VEGF in the eutopic endometrium of the study group were higher than those of the control group (P < 0. 05). In the study group, expression levels of AQP1 and VEGF in the ectopic endometrium from the proliferative stage were significantly higher than those from the secretory stage (P < 0.01). While there were no significant differences in expression levels of AQP1 and VEGF in the eutopic endometrium as well as the endometrium of the control group between the proliferative stage and the secretory stage ( P > 0.05 ). Expression levels of AQP1 in ectopic and eutopic endometria was closely positively correlated with those of VEGF in the study group (r=0.774,p=0.000; r=0.749, P=0.000). While there was no correlation between expression levels of AQP1 and VEGF in the control group (r = -0.015, P =0.946). Conclusion; AQP1 is overexpressed in the ectopic endometrium suggests that AQP1 might be related with adherence and aggression in endometrio-sis. Both AQP1 and VEGF are important in angiogenesis, which might play an important role in the promotion of endometrio-sis.