Cofilin在子宫内膜异位症在位内膜及异位内膜中的表达及意义

摘要

Objective:To explore the expression of cofilin in the eutopic endometrium and ectopic foci of endometriosis, investigate its function in the development and progression of endometriosis. Methods:The eutopic and ectopic endometria sampled from 35 patients who were pathologically proven for EMT in China Medical University Shengjing Hospital from June 2008 to December 2008 were chosen as experimental group (17 cases in proliferative phase, 18 cases in secretory phase, 18 cases in mild group( Ⅰ 、 Ⅱ ), 17 cases in severity group( Ⅲ、 Ⅳ ),and the eutopic endometria from 16 patients without endometriosis were taken as control group(7 cases in proliferative phase, 9 cases in secretory phase). In situ hybridization method was used to examine the expression of cofilin, and the expression level of cofilin in different groups was analyzed statistically. Results:The expression level of cofilin in eutopic endometrium of endometriosis were significantly higher than those in control group ( P ＜0. 01 ). The expression level of cofilin in ectopic foci of endometriosis were significantly higher than those in eutopic endometrium of endometriosis ( P＜0. 01 ). There were statistically significant difference between the levels of cofilin expression in eutopic endometrium and ectopic foci of endometriosis with mild endometriosis and those in eutopic endometrium and ectopic foci of endometriosis with severe endometriosis ( P ＜ 0. 01 ). There were no statistically significant difference between cofilin expression levels in the proliferative phase and the counterparts in secretive phase ( P＞0. 05 ). Conclusions: The unusually elevated levels of cofilin expression in the eutopic endometria of endometriosis may play an important role in the development and progression of endometriosis.%目的:探讨子宫内膜异位症(EMT)在位内膜及异位内膜中候选基因cofilin的表达在EMT发生和发展中的作用.方法:选取我院35例经病理证实为EMT的在位内膜及异位内膜[增殖期17例,分泌期18例;轻度组(Ⅰ、Ⅱ期)18例,重度组(Ⅲ、Ⅳ期)17例]为研究组,以同期16例非EMT患者的子宫内膜标本为正常对照组(增殖期7例,分泌期9例).应用原住杂交方法检测cofilin的表达,并对各组内膜中cofilin表达水平进行统计学分析.结果:研究组的在位内膜cofilin表达水平(2.2569±1.0005)明显高于对照组(1.2525±0.7549)(P<0.01);研究组中EMT异位内膜cofilin表达水平(4.2274±1.2091)明显高于EMT在位内膜(2.2569±1.0005)(P<0.01);研究组中EMT重度组在位内膜及异位内膜cofilin表达水平(分别为2.6941±0.9517及4.5965±1.0353)高于轻度组(分别为1.8439±0.8809及3.8789±1.2846)(P<0.01).对照组内膜组织和研究组中在位内膜及异位内膜的分泌期与增殖期cofilin表达水平比较,差异均无统计学意义(P>0.05).结论:EMT患者在位内膜中cofilin表达水平异常升高在该病的发生、发展中可能起一定作用.