β1,3-N-乙酰氨基葡萄糖转移酶-8在人垂体瘤组织的表达及其对侵袭性的影响

摘要

Objective To explore the expression of β1,3-N-acetylglucosaminyltransferase 8 (β3GnT8) in pituitary adenomas and its influence on the invasive behaviors.Methods A total of 58 cases of pituitary adenoma,including 32 cases in invasive group and 26 cases in non-invasive group,were collected.The expression of β3GnT8,high glycoform of CD147 (HG-CD147) and matrix metalloproteinase (MMP)-2 was determined by immunohistochemical method and Western blotting.Results The β3GnT8 expression rate was significantly higher in the invasive group than in the non-invasive group (87.5％ vs.57.5％,P ＜0.05).Western blotting revealed that the expression of β3GnT8,HG-CD147 and MMP-2 in the invasive group was significantly increased as compared with the non-invasive group (t =2.72,P ＜ 0.05;t ＝ 2.81,P ＜ 0.05;t =2.68,P ＜ 0.05),and with the increased expression of β3GnT8,the expression of HG-CD147 and MMP-2 also increased.There were mutually positive correlations among β3GnT8,CD147 and MMP-2 expression in invasive pituitary adenomas (r =0.78,P ＜ 0.05;r =0.64,P ＜ 0.05;r =0.52,P ＜ 0.05).Conclusion The β3GnT8 expression might relate with the aggressiveness and invasion of pituitary adenomas.It may be an useful maker for predicting invasiveness and provides a new way for the diagnosis and treatment of invasive pituitary adenomas.%目的 探讨β1,3-N-乙酰氨基葡萄糖转移酶-8(β3GuT8)在人垂体腺瘤中的表达及其意义.方法 收集经手术和病理检查结果证实的垂体腺瘤标本58例,其中侵袭组32例,非侵袭组26例,分别采用免疫组织化学和Western blot方法检测侵袭性和非侵袭性垂体腺瘤组β3GnT8、相对高分子质量细胞外基质金属蛋白酶诱导剂(HG-CD147)、基质金属蛋白酶-2(MMP-2)的表达.结果 免疫组织化学结果显示33 GnT8在侵袭组的阳性率为87.5％,明显高于非侵袭组中的57.7％(P＜0.05);Western blot结果显示β3GnT8、HG-CD147、MMP-2在侵袭组表达水平均明显高于非侵袭组(t=2.72,P＜0.05;t =2.81,P＜0.05;t =2.68,P＜0.05),且随着β3GnT8表达量增加,HG-CD147及MMP-2的表达量亦增加,Pearson相关分析显示在侵袭组中β3GnT8、HG-CD147、MMP-2表达两两呈正相关(r =0.78,P ＜0.05;r=0.64,P＜0.05;r=0.52,P＜0.05),在非侵袭组中无明显相关(P＞0.05).结论 β3GnT8在垂体瘤中表达且和垂体腺瘤的侵袭性密切相关.