Objective To observe the expression of transforming growth factor alpha (TGF-α) during liver regeneration after partial hepatectomy and splenic artery ligation,and study the influence of the splenic artery ligation on liver regeneration.Methods Seventy-two male rats were randomly divided into three groups:the sham operation group (A group) given the sham operation;70％ hepatectomy group (B group) given 70％ liver resection;70％ hepatectomy and splenic artery ligation group (C group) griven splenic artery ligation and 70％ liver resection.Each group was divided into subgroups of 24,48,72 h after the operation.The expression levels of TGF-α in liver tissues were measured by immunohistochemical methods.Serum TGF-α levels were determined by enzyme-linked immunosorbent assay (ELISA).Results As compared with A group,the levels of serum TGF-α at 24,48 and 72 h after the operation was significantly incressed in the B group [(242.35 ± 9.33),(163.64 ± 9.22),(206.42 ± 8.42) pg/ml] and C group [(259.00 ± 11.50),(170.77 ± 9.04),(212.12 ± 10.63) pg/ml,P =0.000].The ex pression levels of TGF-α in liver tissues were notably increased at 24,48 and 72 h after the operation in the B group and C group (P =0.000).As compared with B group,the level of serum TGF-α and the ex pression of TGF-α in liver tissues were both increased at 24 h after the operation in C group (P =0.006,P=0.019).There were no significant differences between B group and C group in the level of serum TGF-α and the expression of TGF-α in liver tissues at 48 and 72 h (P =0.163,P =0.074,P =0.194,P =0.096).Conclusion The expression of TGF-α at 24 h after the 70％ hepatectomy was promoted by the splenic artery ligation.The finding demonstrated that the splenic artery ligation can promote liver regen eration at early stage (about 24 h) after 70％ liver resection in rats.%目的 观察脾动脉结扎对部分肝切除大鼠肝脏再生过程中转化生长因子-α(TGF-α)表达的影响,探讨脾动脉结扎对肝再生的影响.方法 将72只雄性SD大鼠随机分为3组:假手术组(A组):假手术处理;70％肝切除组(B组):行70％肝切除;70％肝切除±脾动脉结扎组(C组):结扎脾动脉,其余操作同B组.每组再分为3个阶段检测:术后24h、术后48 h、术后72 h,采用免疫组织化学方法检测肝组织中TGF-α的表达,采用酶联免疫吸附试验(ELISA)检测血清中TGF-α的水平.结果 与A组比较,术后24、48和72 h血清TGF-α水平在B组[(242.35±9.33)、(163.64±9.22)、(206.42 ±8.42) pg/ml]和C组[(259.00±11.50)、(170.77±9.04)、(212.12± 10.63) pg/ml]均明显升高(P=0.000);术后24、48、72 h肝组织中TGF-α的表达水平均明显增高(P =0.000).与B组比较,C组术后24 h血清中TGF-α水平和肝组织中TGF-α的表达水平均增高(P=0.006,P=0.019),在术后48、72 h血清中TGF-α水平和肝组织中TGF-α的表达水平与C组比较差异无统计学意义(P=0.163、P=0.074;P =0.194、P=0.096).结论 结扎脾动脉可促进70％肝切除大鼠术后24 h TGF-α的表达,提示脾动脉结扎可能促进了70％肝切除大鼠术后早期(24 h)肝脏的再生.
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