小鼠胰腺癌进展过程中T细胞亚群的动态变化及其意义

摘要

Objective To investigate whether the distribution of T lymphocyte subsets in the immune organs and tumor microenvironment will change or not during the progression of tumor.Methods In the 1st,2nd,3rd week after the tumor has established,the proportion of CD4 +,CD8 + and CD4 + forkhead box P3 (Foxp3) + cells of CD3 + T lymphocytes in peripheral blood,spleen and tumor were measured by flow cytometer respectively.Results In tumor tissues,the proportions of CD4 + cells were (20.9 ± 3.I)％,(10.1 ±4.9)％,(11.4 ±9.9)％,the proportions of CD8+ cells were (40.6 ±4.9)％,(41.6 ± 3.2) ％,(51.4 ± 11.9) ％,the proportions of CD4 + Foxp3 + cells were (2.34 ± 1.69) ％,(3.34 ± 1.83) ％,(1.36 ± 0.58) ％,all changes had statistically significance (P =0.003,0.026,0.033);in spleen,the proportions of CD4 + cells were (39.5 ± 4.4) ％,(26.5 ± 4.8) ％,(31.8 ±9.4) ％,the proportions of CD4 + Foxp3 + cells were (4.40 ± 0.33) ％,(6.50 ± 2.07) ％,(4.97 ± 2.32)％,these changes had statistically significance (P =0.012,0.005),the changes of CD8 + cells had no significance;in peripheral blood,the proportion of CD4 +,CD8 + and CD4 + Foxp3 + cells had no significant change.Conclusion In the murine pancreatic cancer model,the distribution of T cell subsets had dynamic changes during tumor progression,and the proportions of CD4 +,CD8 + cells had a contrast trend with the ones of CD4 + Foxp3 + cells.Due to the complexity of the varying model of T cell subsets,more studies should be carried out to reveal the mechanism of the anti-tumor immunity.%目的 探讨胰腺癌进展过程中,免疫系统和肿瘤微环境中的T细胞亚群是否会随肿瘤进展而改变.方法 在小鼠胰腺癌模型成瘤后第1、2、3周时,采用流式细胞术检测外周血、脾脏和肿瘤组织中CD4+、CD8+和CD4+叉状头/翅膀状螺旋转录因子(Foxp3)+细胞占CD3+T淋巴细胞的比例.结果 肿瘤组织中,CD4+细胞比例依次为(20.9±3.1)％、(10.1±4.9)％、(11.4±9.9)％;CD8+细胞比例依次为(40.6±4.9)％、(41.6±3.2)％、(51.4±11.9)％、CD4+ Foxp3+细胞比例依次为(2.34±1.69)％、(3.34±1.83)％、(1.36±0.58)％,三类细胞变化差异均有统计学意义(P=0.003、0.026、0.033);脾脏中,CD4+细胞比例依次为(39.5±4.4)％、(26.5±4.8)％、(31.8±9.4)％;CD4+ Foxp3+细胞比例依次为(4.40±0.33)％、(6.50 ±2.07)％、(4.97±2.32)％,其变化差异有统计学意义(P=0.012、0.005),CD8+细胞的变化差异无统计学意义;外周血中,CD4+、CD8+和CD4+ Foxp3+细胞比例的变化差异均无统计学意义.结论 小鼠胰腺癌模型中T细胞亚群的分布会随肿瘤进展发生动态变化,且CD4+、CD8+细胞与CD4+ Foxp3+细胞的变化趋势相反.由于各细胞亚群的变化模式较为复杂,仍需进一步研究来探明其机制.