接枝肝素的聚己内酯支架临床适用性

摘要

目的 观察接枝肝素的聚己内酯支架的体外特征,探讨其作为组织工程血管用支架的可行性.方法 使用电纺丝技术将接枝肝素的聚己内酯纺成口径约为3 mm的管状支架.扫描电镜明确其表面特征,测量接触角明确亲水性,测量爆裂压明确其力学性能,压汞法测量其孑L隙率大小,测定活化部分凝血酶原时间评估其抗凝效果,采用噻唑蓝(MTT)比色法明确其细胞毒性,皮下埋植明确支架与机体组织的相容性.结果 扫描电镜提示纺丝直径约为0.5 ～10.0 μm,静态接触角为(73.6±0.7).,爆裂压为(1 558.5±16.3)mmHg(1 mmHg =0.133 kPa),孔隙率为83％,体外具有明显抗凝效果,无明显细胞毒性,与机体组织相容性好.结论 接枝肝素的聚己内酯可作为组织工程血管用支架材料.%Objective To investigate the characterization of electrospun heparin-bonded polycaprolactone (hPCL) in vitro and to evaluate the possibility of heparin-bonded polycaprolactone (PCL) as scaffold of tissue-engineered blood vessel (TEBV).Methods Heparin was bonded to PCL and PCL was electrospun to scaffold in 3mm diameter to construct hPCL scaffold.hPCL was evaluated by scanning electron microscopy (SEM) to determine the surface configuration.Contact angle of hPCL was measured to obtain the hydrophilicity.Mechanical property of hPCL was investigated.Porosity of hPCL was determined by mercury intrusion porosimetry.Activated partial thromboplastin time (APTT) of hPCL was determined in vitro.methyl thiazol tetrazolium (MTT) was used to evaluate the cytotoxicity of hPCL.HPCL were implanted subdermally to evaluate the histocompatibility.Results Scanning electron microscopy (SEM)showed the diameter of fiber of hPCL was from 0.5 μm to 10.0 μm.Static contact angle was (73.6 ± 0.7) degree.The burst pressure was (1 558.5 ± 16.3) mmHg (1 mmHg =0.133 kPa).Porosity of hPCL was 83％.APTT test showed that hPCL scaffold exhibit obvious anticoagulation in vitro.hPCL was non-toxic for cells and acceptable histocompatibility.Conclusion hPCL can be used as scaffold of TEBV.