HIV-1流行毒株HIV DNA的研究

摘要

目的了解HIV-1流行毒株中HIV DNA的变异特性。方法用逆转录PCR(RT-PCR)，长片段PCR(Long-Distance PCR LD-PCR)、分子克隆、杂交和测序等对63例HIV-1感染者外周血单个核细胞(PBMC)HIV DNA进行研究。结果 57例标本可明确定型，其中B亚型43例，C亚型5例，E亚型9例。57例中31例既存在完整又存在不完整的HIV-1 DNA，19例只存在不完整的HIV-1 DNA片段，7例只有完整的HIV-1 DNA，缺失的最大范围为7 759个碱基，最小范围71个碱基，以多片段缺损最为常见。结论 HIV DNA基因片段变异在HIV感染和流行传播中是非常重要的。%Objective To study the characteristics of variation of HIV DNA in prevalent HIV-1 strains. Methods HIV DNA, extracted from peripheral blood mononuclear cell(PBMC) in 63 patients infected with HIV, was investigated by reverse-transcript PCR (RT-PCR), Long-Distance PCR( LD-PCR), molecular cloning hybridization and sequencing, respectively. Results The subtypes were identified in 57 samples. Subtype B was identified in 43 cases,subtype C in 5 and subtype E in 9. It was found that both full-length and defective HIV DNA in 31 out of 57 cases, defective HIV DNA in 19 cases and full-length HIV DNA in 7 cases. The deleted regions of genome were from 71 to 7 759 nucleotides. Conclusion The variation of gene segments of HIV DNA plays an important role in the infection and epidemic of HIV.