吉林省麻疹野病毒的基因型别和基因特征

摘要

Objective To study the genotype and gene characterization of measles wild viruses circulated in Jilin provinces,and to provide scientific evidences for setting down controlling and preventing strategy and measures.Methods 38 strains of measles virus isolated in 2001-2006 were genotyped by RT-PCR-RFLP,some strains of measles virus in Jilin province were chosen for the phylogenetic analysis and for the homology analysis of nucleotide and amino acid sequences. Results All the 38 strains of measles virus were identified as H1 genotype by RT-PCR-RFLP,and 29 strains of them were identified further as H1a by sequence analysis. The homology of nucleofide was 88.0%-89.4% and the homology of amino acid was 91.8%-92.7% .The average diversity was less than 1.4% .Conclusion The measles virus of H1a genotype was the circulating virus within recent years in Jilin province.There were the same measles virus strains circulating and transmitting at different years and also the different H1a measles virus strains co-circulating at the same year.There were the same transmission chain caused by the same measles virus with other provinces.%目的 阐明吉林省流行麻疹野病毒的基因型别和基因特征,为制定麻疹防控策略措施提供科学依据.方法 用逆转录-聚合酶链反应.限制性片段长度多态性分析(RT-PCR-RFLP)方法对2001-2006年分离的38株麻疹病毒进行基因分型;选择麻疹病毒代表株RT-PCR扩增N基因C-末端450个核苷酸,对比野毒和疫苗株核苷酸和氨基酸同源性,并构建N基因亲缘关系树.结果 经RT-PCR-RFLP基因定型,38株麻疹病毒分离珠均为H1基因型;对其中的29株进一步进行N基因C-末端450个核苷酸的序列测定和分析证实均为H1基因型的H1a亚型.吉林分离株与我国麻疹疫苗S191株450个核苷酸同源性为88.0%～89.4%,所提示氨基酸的同源性为91.8%～92.7%;吉林省内分离株核苷酸平均变异小于1.4%.结论 H1a基因亚型为吉林省近年来流行的麻疹野病毒绝对优势基因亚型.不同年份存在相同麻疹病毒的持续循环传播,同一年份也存有H1a亚型内不同病毒的共循环;与其他省之间存在相同麻疹病毒引起的传播链.