HIV/HCV共感染者NK细胞数量和功能的研究

摘要

Objective To study the impacts of HIV/HCV co-infection to NK cells by investigating the changes of frequencies and functions of NK cells in HIV/HCV co-infected patients. Methods Frequencies and counts of NK cells were measured in patients with HIV mono-infection, HCV monoinfection, HIV/HCV co-infection and health control (HC) group by flow cytometer ( FCM ). After stimulated with PMA and K562 cells, PBMCs were examined the proportion of IFN-γ+ NK cells by FCM. Proportion of killed K562 cells were detected to analyze the killing functions of NK cells. Results The frequencies of NK cells, the percentages of IFN-γ+ NK cells as well as the functions of NK cells killing the K562 cells in HIV/ HCV co-infection, HIV mono-infection and HCV mono-infection groups were all lower than those of HC group significantly, the absolute counts and killing functions of NK cells in co-infection group were significantly lower than those of HIV or HCV mono-infection group. Conclusions The counts and functions of NK cells were affected more in HIV/HCV co-infections than those in HIV or HCV mono-infection.%目的 研究HIV/HCV共感染者的NK细胞数量和功能的变化，探讨HIV/HCV共感染对NK细胞的影响。方法 应用流式细胞术(FCM)检测HIV/HCV共感染者(n=18)、单纯HIV感染者(n=20)、单纯HCV感染者(n=30)及健康对照(n=18)的NK细胞数量，用PMA和K562细胞刺激外周血单个核细胞(PBMC)、流式细胞术检测NK细胞分泌IFN-γ的能力，并用流式细胞毒性分析法检测NK细胞对K562细胞的杀伤效率，对各组NK细胞的功能进行分析和比较。结果 HIV/HCV共感染者、单纯HIV感染者、单纯HCV感染者的NK细胞频率、NK细胞分泌IFN-γ的能力以及对K562细胞的杀伤能力都显著低于健康对照；HIV/HCV共感染者的NK细胞绝对值和对K562细胞杀伤能力显著低于HIV和HCV单纯感染者。结论 HIV/HCV共感染对NK细胞数量和功能的影响较单纯HIV和HCV感染更加严重。