衢州市无偿献血者中隐匿性HBV感染的基因型别及突变类型分析

摘要

Objective To assess the molecular biological characteristics of occult hepatitis B virus (HBV) infection in blood donors in Quzhou.Methods A total of 24 178 blood samples from the free-of-charge blood donors were enrolled in the study,all of donations were tested by two different HBsAg ELISA.The occult HBV infection (OBI) was determined by DNA analysis among the HBsAg negative samples.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 24 020 HBsAg negative samples out of 24 178 blood donors,15 were HBV DNA positive.The occult HBV infection rate was 0.62‰ (15/24 020).Among the 15 samples,9 were genotype C (60％) and 6 genotype B (40％).In addition,a specific mutation in "a" epitope was observed in 11 OBI virus strains by amino acid analysis.Conclusion There was occult HBV infection among blood donors in Quzhou,which is probably associated with the gene mutation of HBV virus.%目的 了解衢州市无偿献血者隐匿性乙肝病毒感染情况及病毒的分子生物学特征.方法 对衢州市中心血站24 178例无偿献血者血液用北京万泰和Sorin公司生产的ELlSA试剂盒进行HBV的初检和复检,对HBsAg阴性标本进行HBV DNA检测,从而检出隐匿性乙肝病毒感染者,再对HBV DNA 阳性标本进行 S 基因片段序列分析和氨基酸突变分析,从而了解本市无偿献血者隐匿性乙肝病毒感染情况和病毒分子生物学特征,探讨隐匿性乙肝病毒感染的可能机制.结果 24 178例无偿献血者标本中,158例HBsAg阳性,24 020例HBsAg阴性标本中,15例HBV DNA阳性,隐匿性HBV感染比例为0.62‰(15/24 020),其中基因C型9例(60％),基因B型6例(40％);S基因“a”表位氨基酸突变分析显示有11例隐匿性HBV感染病毒株在“a”表位发生突变.结论 衢州市无偿献血者中存在一定比例隐匿性乙肝病毒感染,隐匿性乙肝病毒感染与病毒基因突变有相关性.