一种快检试剂对不同基因型诺如病毒的检测评价

摘要

Objective To evaluate the efficiency of a rapid immunochromatographic test kit for noroviruses of different genotypes in fecal specimens.Methods Between April 2011 and May 2015,101 norovirus-positive fecal specimens were collected from patients with diarrhea.Noroviruscs of genogroup Ⅰ (G Ⅰ) or Ⅱ (G Ⅱ) were detected using the real time RT-PCR.The capsid genes of noroviruses were amplified using the RT-PCR,and genotypes were identified through the phylogenetic analysis.The results of real-time RT-PCR and genotyping were employed as the reference,then the rapid immunochromatography test kit (RIDA(R) QUICK Norovirus) was evaluated.Results Among 101 specimens,8 strains of GⅠ and 93 strains of G Ⅱ were identified by real time RT-PCR,whereas 1 strains of GⅠ and 57 strains of G Ⅱ were identified by RIDA(R) QUICK Norovirus,with the coincidence rate of 57.4％.RIDA(R) QUICK Norovirus could detect GⅠ.9,G Ⅱ.3,G Ⅱ.4,G Ⅱ.6,G Ⅱ 7,G Ⅱ.13,G Ⅱ.17,G Ⅱ.21 and G Ⅱ.N,and it failed to detect GⅠ.2,GⅠ.6,GⅠ.N and G Ⅱ.2.The detection rates for G Ⅱ.4 strains and new G Ⅱ.17 variants were 77.8％ (28/36) and 38.1％ (8/21) respectively.Conclusion This assay was effective for common GⅡ noroviruses,but less reliable for new G Ⅱ.17 variants.%目的 评价一种快检试剂对不同基因型诺如病毒的检测效果.方法 收集2011年4月到2015年5月诺如病毒核酸阳性的腹泻患者粪便标本101份,用荧光定量RT-PCR检测GⅠ/GⅡ组诺如病毒核酸.用逆转录聚合酶链反应(RT-PCR)扩增衣壳蛋白区基因,测序、进化分析确定基因型.以荧光定量RT-PCR检测和基因型分型结果为参照,对德国拜发公司免疫层析法快速检测试剂(RIDA(R)QUICK Norovirus)进行评价.结果 101份标本中,荧光定量RT-PCR检测出8份GⅠ,93份GⅡ.RIDA(R)QUICK检出1份GⅠ,57份GⅡ,符合率57.4％.RIDA(R)QUICK可检出GⅠ.9、GⅡ.3、GⅡ.4、GⅡ.6、GⅡ7、GⅡ.13、GⅡ.17、GⅡ.21和GⅡ.N,不能检出GⅠ.2、GⅠ.6、GⅠ.N和GⅡ.2.RIDA(R)QUICK对GⅡ.4型诺如病毒的检出率较高(77.8％,28/36),但对新的GⅡ.17变异株检出率较低(38.1％,8/21).RIDA(R)QUICK阳性的条带深浅度与荧光定量RT-PCR平均Ct值存在相关性(rs=0.498).结论 RIDA(R) QUICK能检出GⅡ组诺如病毒的常见基因型,但对新出现的GⅡ.17变异株检出效果较差.