安徽省诺如病毒胃肠炎暴发的分子病原学特征研究

摘要

Objective To study the genotype and phylogenetic characterization of the pathogens associated to the epidemic outbreak of acute gastroenteritis.Methods A total of 19 anal swab and feces samples from acute gastroenteritis outbreak in a junior middle school were collected in November 2014,Anhui province.Norovirus (NoV) nucleic acid was detected by Real-time PCR method,and the partial capsid gene of the all positive specimens were amplified by conventional RT-PCR and sequenced.Phylogenetic tree was constructed by the Neighbor-Joining method based on partial capsid gene sequences of norovirus to perform phylogenetic analysis.Results Of the 19 specimens,12 (12/19) were positive for NoV,and the positive samples of those were sequenced.Phylogenetic analysis showed that 11 strains were belong to genotype GⅡ.2,and one strain was belong to genotype GⅡ.6 norovirus.The nucleotide identity of the partial capsid gene sequences were 95.60％ between 11 genotype GⅡ.2 strains identified and Melksham strain of norovirus.Conclusion The epidemic outbreak of acute gastroenteritis from Anhui province was caused by genotype GⅡ.2 and GⅡ.6 norovirus co-infection,and genotype GⅡ.2 norovirus was predominant strains in this outbreak.%目的 明确安徽省一起学校急性胃肠炎暴发疫情的病原基因型别及分子遗传进化特征.方法 采集2014年12月安徽省一起学校暴发的病例肛拭子(粪便)标本19份,采用荧光定量RT-PCR检测诺如病毒基因,并对阳性标本进行传统RT-PCR扩增、基因测序和鉴定基因型别,与国内外参考株进行序列比对和构建进化树.结果 诺如病毒荧光定量RT-PCR显示19份标本中12份阳性,基因序列比对和进化分析结果显示,11个为GⅡ.2基因型,1个为GⅡ.6基因型.结论 安徽省该起学校胃肠炎暴发疫情主要由GⅡ.2型诺如病毒引起,同时存在GⅡ.6型诺如病毒混合感染.