Objective To understand the novel bat-borne hantavirus on Yunnan province.Methods Eighty-four bats were collected from Puer,Yunnan from July to August in 2016.A hantavirus,named as DodeHV,was identified in Pomona Roundleaf Bat by high-throughput sequencing technology.Nested or Semi-nested reverse transcription-polymerase chain reaction was used to confirm and screen DodeHV,as well as to amplify its genome sequence by specific primers.Then homology and phylogenetic analysis of all three segments were conducted by using software MegAlign and MEGA 6.0.Results All the positive individuals are Pomona Roundleaf bats by PCR in this study and 4 out of 67 (5.97％) Pomona Roundleaf bats were positive.The complete ORF of DodeHV S Segment,the most sequence of M and the complete sequence of L segment were obtained.It shared the highest homology with the strain XSVVN1982B4 found on Phu Tho Province,Viet-Nam in 2013,the nucleotide sequence identities of S,M,L segment compared with XSV was 79.0％,79.2％,79.9％ respectively,and its amino acid sequence identities was 93.4％,94.8％,96.6％ respectively.Meanwhile,phylogenetic analysis showed that DodeHV was also closely related to strain XSV-VN1982B4.Conclusions The discovery of DodeHV enriched the virus reservoir of our country which is meaningful to public health.It is indicated that there is potential risk of bat-borne DodeHV at cross-border infection at frontier.%目的 探索云南地区蝙蝠携带的新型汉坦病毒.方法 本研究于2016年7～8月在云南普洱地区采集到84只蝙蝠,利用病毒宏基因组学分析蝙蝠携带病毒种类,采用巢式或半巢式PCR鉴定汉坦病毒.采用MegAlign进行同源性分析,使用MEGA6.0进行序列比对及系统进化分析.结果 本研究在小蹄蝠体内发现了1株汉坦病毒,命名为DodeHV,阳性标本均为小蹄蝠,通过PCR筛查发现在小蹄蝠中阳性率为5.97％ (4/67).本实验获得了DodeHV S节段完整编码区序列和L节段的全部序列,以及M节段的部分序列.序列分析发现DodeHV与2013年在越南富寿省发现的XSV-VN1982B4毒株同源性最高,S、M、L节段的核苷酸序列相似度分别为79.0％、79.2％和79.9％,对应的氨基酸序列相似度分别为93.4％、94.8％和96.6％.系统进化分析也显示它们的进化关系最近.结论 本研究在云南小蹄蝠体内发现了1株新的汉坦病毒DodeHV,进一步丰富了我国的蝙蝠病毒库,同时也提示我国边境地区蝙蝠携带该病毒传播的风险,具有一定的公共卫生学意义.
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