Klotho基因多态性与老年高血压患者靶器官损害的相关性分析

摘要

目的 通过分析klotho G395A,F352V,C370S三个位点的等位基因与单核苷酸多态性(SNP)在老年性高血压人群中的分布情况,探究klotho基因对高血压患者造成靶器官损害的相关特性.方法 选取2013年1月~2016年1月于河北港口集团有限公司港口医院神经内科老年高血压患者104例,其中男性54例,女性50例,平均年龄(67.32±7.32)岁.按是否合并冠状动脉粥样硬化性心脏病(冠心病,CHD)分为两组,即高血压合并CHD组(n=57,男性30例,女性27例)和高血压未合并CHD组(n=47,男性24例,女性23例).再按是否发生肾损害分为高血压合并肾损害组(n=66,男性35例,女性31例)和高血压未合并肾损害组(n=38,男性19例,女性19例),分别统计各组患者临床资料及klotho的等位基因的分布情况,分析其与心、肾靶器官损害的相关性.结果 在合并CHD和未合并CHD两组患者中,合并CHD组患者年龄较高,而性别,糖尿病及血脂等方面,则无显著差异(P>0.05);对klotho基因G395A SNP的三个等位基因GG,GA,AA的比较中,合并CHD和未合并CHD两组中患者无显著差异;性别比较显示:男性患者AA基因型在合并CHD组中显著高于未合并CHD组,差异有统计学意义(P<0.05).klotho基因F352V SNP中,FV基因型的频率在高血压并发CHD组中较未并发CHD的患者更低,在男性分层中这一差距依然存在,差异有统计学意义(P<0.05);klotho基因SNP C370S的各个等位基因CC,CS,SS在两组中均无显著差异,按性别分层后仍无显著差异(P>0.05).结论 男性高血压患者伴klotho基因G-395A SNP AA等位基因易并发CHD,而携带F352V FV等位基因的男性高血压患者患CHD的概率更低;高血压合并肾损害与klotho基因的相关性不明显.%Objective To investigate the correlation between Klotho gene and target organ injury through analyzing the distribution of alleles at 3 locus (G395A, F352V, C370S) of Klotho gene and single nucleotide polymorphism (SNP) in elderly patients with hypertension. Methods The patients (n=104, male 54, female 50 and average age=67.32±5.85) were chosen from the Department of Neurology of Port Hospital of Hebei Port Group Co., LTD from Jan. 2013 to Jan. 2016. All patients were divided, according to whether or not with complicated coronary heart disease (CHD), into hypertension+CHD group (n=57, male 30 and female 27) and hypertension without CHD group (n=47, male 24 and female 23). The patients were again divided, according to whether or not with renal damage, into hypertension+renal damage group (n=66, male 35 and female 31) and hypertension without renal damage group (n=38, male 19 and female 19). The clinical materials were collected from all groups. The distribution of alleles of Klotho gene was respectively counted and the correlation between the alleles and target organs (heart and kidney) was analyzed. Results The age was elder in hypertension+CHD group than that in hypertension without CHD group, and sex, diabetes and blood sugar had no significant difference between 2 groups (P>0.05). The comparison in 3 alleles (GG, GA, AA) of G395A SNP of Klotho gene had no significant difference between 2 groups. The comparison in sex showed that AA genotype in male patients were significantly higher in hypertension+CHD group than that in hypertension without CHD group (P<0.05). The frequency of FV genotype of F352V SNP of Klotho gene was lower in hypertension+CHD group than that in hypertension without CHD group, and the difference existed in male hierarchy (P<0.05). The alleles (CC, CS, SS) of SNP C370S of Klotho gene had no significant difference between 2 groups and also in sex hierarchy (P>0.05). Conclusion Male patients with hypertension carrying AA allele of G-395A SNP of Klotho gene are commonly complicated by CHD, and those carrying FV allele of F352V of Klotho gene have a lower probability of CHD. The correlation between hypertension complicated by kidney damage and Klotho gene is not significant.