β-arrestin2基因多态性与湖南省汉族海洛因成瘾者美沙酮维持治疗反应的关联分析

摘要

目的 研究β-arrestin2 (ARRB2)基因的3个单核苷酸多态性(SNP)位点rs3786047、rs1045280、rs2036657在湖南省汉族海洛因成瘾者中的分布及其与美沙酮维持治疗反应的相关性.方法 从湖南省美沙酮维持治疗门诊中随机抽取4个门诊,以汉族美沙酮维持治疗者作为研究对象,收集研究对象的一般社会经济学特征、相关吸毒史和美沙酮治疗史资料,测定ARRB2基因多态性位点的基因型以分析SNP位点与治疗反应的关系.结果 ARRB2基因rs3786047、rs1045280、rs2036657 SNP位点在不同治疗反应组基因型分布频率符合Hardy-Weinberg遗传平衡.在治疗反应好与差两组间,rs3786047(x2=0.486 2,P=0.784)、rs1045280(x2=1.591 9,P=0.451)、rs2036657(x2=1.061 5,P=0.588)的基因型频率分布和等位基因频率分布差异无统计学意义.结论湖南省汉族美沙酮维持治疗人群中尚未发现ARRB2基因单核苷酸多态位点rs3786047、rs1045280、rs2036657与治疗反应有关联关系.%Objective To study the distributions of three single nucleotide polymorphisms (SNPs) in β-arrestin2 (ARRB2) which including rs3786047,rs1045280 and rs2036657 and to elucidate the relationship between these SNPs and response to methadone maintenance treatment (MMT) among heroin-dependent patients of Han ethnicity population in Hunan.Methods Han MMT patients were recruited in four random-chosen MMT clinics from Hunan province.Demographics,history of drug-use and MMT were recorded.ARRB2 SNPs were genotyped to determine the association between SNPs and response to MMT.Results Distributions of the three SNPs were in Hardy-Weinberg equilibrium in both groups (responders vs.non-responders).There was no statistical significance in the distribution frequency of genotype on rs3786047 (x2=0.486 2,P=0.784),rs1045280 (x2=1.591 9,P=0.451) and rs2036657 (x2=1.061 5,P=0.588) in ARRB2 among the responders or the non-responders.Conclusion Associations between the ARRB2 genotypes,rs3786047,rs1045280 and rs2036657,and MMT response in Han MMT patients in Hunan province did not appear.