肿瘤坏死因子-β基因多态性与特发性扩张性心肌病的相关性分析

摘要

目的 探讨肿瘤坏死因子(TNF)-β基因型的多态性与特发性扩张性心肌病(IDC)的相关性.方法 在吉林大学第一临床医院,选择86例IDC患者为IDC组、95例体检健康者为对照组,用ELlSA法检测血清TNF-β蛋白水平,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析法检测TNF-β基因多态性变化.结果 IDC组TNF-β蛋白水平[(1.876±1.013)μg/L]高于对照组[(1.018±0.645)μg/L],组间比较差异有统计学意义(t=2.67,P<0.01);TNFμ*2等位基斟频率[63.4%(109/172)1高于对照组比[47.9%(91/190)],组间比较差异有统计学意义(x2=6.78,P<0.05),其比值比(伽)值为1.88.结论 TNFμ*2等位基因的多态性与IDC的易感性有关,可能是IDC的易感基因之一.%objective To study the relationships between TNF-β gene type in idiopathic dilated cardiomyopathy (IDC). Methods Eighty-six IDC patients were chosen as IDC group and 95 cases unrelated healthy people as control group in the First Hospital of Jilin University in 2008. The levels of TNF-β protein were detected with Enzyme-linked immunosorbent assay(ELISA ). TNF-β polymorphism were analyzed by polymerase chain reaction-restriction fragment length polymorphisms(PSR-RFLP). Results The levels of TNF-β protein in IDC patients[(1.876±1.013) μ/L] were significantly higher than controls[(1.018±0.645)μg/L], the difference being statistically significant (t=2.674,P<0.01). The frequency of TNFβ*2 genotypes in IDC patients[63.4%(109/172] was significantly higher than controls[47.9%(91/190)], the difference being statistically significant(OR=1.88, X2=6.78, P<0.05). Conclusions The TNFβ*2 allele might related to idiopathic dilated cardiomyopathy (IDC), can also be considered as one of susceptible genes of IDC