阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及Bcl-2和Bax表达的影响

摘要

目的：探讨阿胶对围绝经期大鼠卵巢颗粒细胞凋亡及其相关基因Bcl-2和Bax表达的影响。方法：选用4月龄雌性大鼠为正常对照组，14月龄阴道细胞学表现为动情期延长的雌性大鼠为衰老大鼠模型。模型动物随机分别给予阿胶或戊酸雌二醇片治疗。采用原位末端核苷酸标记法（TUNEL）检测卵巢细胞凋亡，免疫组化法检测细胞凋亡相关因子Bcl-2，Bax。结果：阿胶高、中、低剂量组卵巢细胞凋亡阳性表达与模型组比较有显著性差异，同时可使卵巢凋亡基因Blc-2表达增强，促凋亡基因Bax表达减弱，Bcl-2／Bax比例增加。结论：阿胶通过抑制Bax蛋白的表达，上调Bcl-2蛋白表达，抑制卵巢颗粒细胞凋亡，进而改善卵巢功能。%Objective:To study the effects of Colla Corii Asini on ovarian granulosa cells apoptosis and expression of Bcl-2 and Bax in perimenopausal rats.Methods:SD female rats of 4 months old were set to be the normal control group and SD female rats of 14 months old whose oestrus elongation in colpocytology appearance were set as the senile female rat model.These rats were divided into 5 groups randomly, as follows:high dose Colla Corii Asini group, middle dose Colla Corii Asini group, low dose Colla Corii Asini group, estradiol valerate group, model group.After 8 weeks in the intervention period, ovarian apoptosis and the expression of ovarian granulosa cell Bcl-2, Bax were measured by TUNEL and immunohisto-chemistry methods.Results:High and middle dose Colla Corii Asini groups could de-crease apoptosis in ovarian granulose cells (P<0.05), and could improve the expression of Bcl-2 in the ovaries and weakened the ex-pression of Bax, which then increase the ratio of Bcl-2/Bax.Conclusion:Colla Corii Asini could delay ovarian granulose cells apoptosis through inhibiting the expression of Bax, increasing the expression of Bcl-2 during perimenopausal period, which might be one reason of Colla Corii Asini postponed the ovarian failure and cured perimenopausal syndrome.