自然杀伤细胞活化性受体DNAM-1和NKG2D的配体在结肠癌中的表达

摘要

目的 探讨自然杀伤细胞活化性受体DNAM-1和NKG2D的配体在结肠癌中的表达.方法 收集2010年6月至2011年1月第四军医大学唐都医院收治的42例结肠癌患者的结肠组织标本以及距肿瘤边缘≥10 cm的正常肠黏膜组织,采用免疫组织化学法检测结肠癌组织及正常肠黏膜组织中自然杀伤细胞活化性受体DNAM-1和NKG2D的配体CD155、CD112和MICA/B的表达情况.采用流式细胞仪检测4种来自Duke's A、B、C和D期的结肠癌细胞系SW116、SW480、SW620和Colo205的CD155、CD112和MICA/B的表达情况.3种配体的表达水平与临床病理因素之间的关系采用Mann-Whitney U检验、x2检验及Fisher确切概率法分析.结果 正常肠黏膜组织可见CD155弱阳性表达,未见CD112及MICA/B的表达.在结肠癌组织中3种配体的表达水平均显著增高,CD155、CD112和MICA/B的表达率分别为81.0％、52.4％和47.6％.3种配体的表达水平与患者的性别、淋巴结转移、肿瘤分化程度和Duke's分期无关(P＞0.05).CD155、CD112和MICA/B在结肠癌细胞系SW116、SW480、SW620和Colo205中的总体表达率分别为88.9％、67.4％和42.3％；CD155的总体表达率显著高于CD112和MICA/B的总体表达率(F =23.17,P ＜0.05).结论 CD155、CD112和MICA/B在结肠癌组织及4种结肠癌细胞系中均有表达,其中CD155的表达水平较高.%Objective To investigate the expression of ligands of DNAM-1 and NKG2D in the colonic cancer.Methods The colonic cancer tissue and adjacent normal colonic tissues were collected from 42 colonic cancer patients who were admitted to the Tangdu Hospital of Fourth Military Medical University from June 2010 to January 2011 were retrospectively analyzed.The expressions of CD155,CD112 and MICA/B in the colonic cancer tissues and the normal colonic tissues were detected by immunohistochemistry.The expressions of CD155,CD112 and MICA/B in the colonic cell line SWll6,SW480,SW620 and Colo205 in the Duke's A,B,C and D phases were detected by cell cytometry.The relationship of the expressions of the 3 ligands and the clinicopathological parameters was analyzed using the Mann-Whitney U test,chi-square test and Fisher exact probobility.Results Week expression of CD155 was found in the normal colonic tissues,while the expressions of CD112 and MICA/B were not found.In the colonic cancer tissues,the expressions of CD155,CD112 and MICA/B were 81.0％,52.4％ and 47.6％,which were significantly increased.The expressions of CD155,CD112 and MICA/B were not correlated with the gender,tumor differentiation,lymph node metastasis and Duke's staging (P ＞ 0.05).The overall expression rates of CD155,CD112 and MICA/B in the colonic cancer cell line SWll6,SW480,SW620 and Colo205 were 88.9％,67.4％ and 42.3％,respectively.The overall expression of CD155 was significantly higher than CD112 and MICA/B (F =23.17,P ＜ 0.05).Conclusion CD155,CD112 and MICA/B express in the colonic cancer tissues and colonic cancer cell line SW116,SW480,SW620 and Colo205,and the expression of CD155 is the highest.