补肾法对人腰椎间盘退变中水通道蛋白AQP1、AQP3表达的影响

摘要

Objective To observe the expression of aquaporin 1 (AQP1) and aquaporin 3 (AQP3) in the progress of intervertebral discs degeneration,and investigate the expression change before and after tonifying kidney treatment, to study its mechanism. Methods 24 cases with prolapse of lumbar intervertebral disc were selected to undertake operation, and randomly divided into control group (12 cases, direct surgery) and treatment group (12 cases, surgery after 1 month therapy of tonifying kidney method) , 12 cases with spine trauma required surgery healers as normal group. The mRNA level of AQP1 and AQP3 was analyzed by real time reverse transcriptim polymerase chain reaction (RT-PCR). The expression change of AQP1 and AQP3 were observed by RT-PCR and western blot after tonifying kidney method. Results Result of immunohisto-chemistry showed the expression of AQP1, AQP3 was more significant between the normal group and the control group ( P < 0.05). When after intervertebral discs degeneration happened, the expression of AQP1 and AQP3 were decreased gradually at the mRNA and protein level compared to the control group ( P <0.05). However, following the using tonifying kidney method, group, the expression of AQP1 and AQP3 were increased gradually at the mRNA and protein level ( P <0.05) , compared to the control group. Conclusion The progress of intervertebral discs degeneration was correlated to the AQP1 and AQP3, the expression of AQP1 and AQP3 were decreased gradually at the mRNA and protein level, the expression of AQP1 and AQP3 were increased gradually at the mRNA and protein level after treatment. It suggests that the tonifying kidney method is involved in the up-regulate the expression of AQP1 and AQP3.%目的 研究人体腰椎间盘退变过程中水通道蛋白AQP1、AQP3表达的变化,并观察补肾法治疗前后AQP1、AQP3表达的变化并探讨其作用机制.方法 选取符合腰椎间盘突出症手术者24例,随机分为对照组(12例,直接手术)和治疗组(12例,补肾法治疗1个月后手术),脊柱创伤需要接受手术治疗者12例作为正常组.荧光定量RT-PCR法、Western blot法检测人体腰椎间盘组织退变前后AQP1、AQP3 mRNA和蛋白表达水平的变化；观察补肾法治疗前后AQP1、AQP3mRNA和蛋白的表达变化.结果 对照组AQP1、AQP3 mRNA及蛋白表达水平较正常组明显降低,差异有统计学意义(P＜0.05)；治疗组AQP1、AQP3 mRNA及蛋白表达水平较对照组增加,差异有统计学意义(P＜0.05).结论 腰椎间盘发生退变时,AQP1、AQP3基因和蛋白的表达水平均下降,经过补肾法治疗后AQP1、AQP3的表达有所增加,提示补肾法治疗腰椎间盘退变可能与AQP1、AQP3的表达上调有关.