自拟地圣珠水煎剂对血管性痴呆小鼠海马IL-1β、IL-6的影响

摘要

目的 观察自拟地圣珠水煎剂对血管性痴呆(VD)模型小鼠脑海马组织中IL-1β、IL-6的影响.方法 45只小鼠随机分为假手术组、模型组、中药组各15只,后2组采用夹闭小鼠双侧颈总动脉造成脑缺血再灌注脑损伤模型,而后中药组给予地圣珠水煎剂,模型组、假手术组给予等量生理盐水,2周后采用ELISA法测定小鼠模型脑海马组织中IL-1β、IL-6的水平.结果 与假手术组相比,模型组小鼠脑海马IL-1β、IL-6水平显著升高(P<0.05),而与模型组比较,中药组IL-1β、IL-6水平明显降低(P<0.05).结论 地圣珠水煎剂能够通过下调脑海马组织中IL-1β、IL-6的水平,干扰炎性反应继发损伤过程,从而达到对VD的满意干预效果.%Objective To observe the effects of Chinese compound recipe Dishengzhu on the level of IL-1β and IL-6 in brain hippocampal tissue of mouse models with vascular dementia. Methods 45 mouses were divided into 3 groups randomly and averagely including the pseudo-operation group, the model control group and the Dishengzhu group,duplicated the synthet-ic vascular dementia mouse models through transient occlusion of bilateral carotid artery in the model control group and thernDishengzhu group. The Chinese medicine group is administer] respectively with the compound recipe Dishengzhu while other groups with the normal saline only. The level of IL-1β and IL-6 in brain hippocampal tissue of mouse models was observed by the means of ELISA method 2 weeks later. Results Compared with the pseudo-operation group, the level of IL-1β and IL-6 in brain hippocampal tissue of mouses was improved apparently in the model control group( P < 0. 05 ). Compared with the model control group, the level of IL-1β and IL-6 was markedly decreased in brain hippocampal tissue of mouses in Dishengzhu group ( P < 0. 05 ). There were remarkable differences. Conclusion The curative effect of Chinese compound recipe Dishengzhu was remarkable by the mean of decreasing the level of IL-1β and IL-6 in brain hippocampal tissue and restraining inflammatory response.