PPARγC161→T、α-内收蛋白 Gly460 Trp 基因多态性与原发性高血压的关系

摘要

目的：探讨过氧化物酶体增殖物活化受体γ（ PPARγ） C161→T及α-内收蛋白Gly460 Trp基因多态性与原发性高血压（ EH）的关系。方法原发性高血压患者（高血压组）262例与正常人群270例（健康对照组）为研究对象，应用聚合酶链反应-限制性片段长度多态性方法对PPARγ基因及α-内收蛋白Gly460 Trp基因多态性位点进行分析，统计2种基因多态性频率并进行比较。结果高血压组PPARγC161→T 基因型分布情况为TT 型10例（3痧．82％），TC型70例（26．72％），CC型182例（69．46％），T型等位基因为90例次（17．18％），C型等位基因为434例次（82．82％）；健康对照组PPARγC161→T基因型分布情况为TT型17例（6．30％），TC型119例（44．07％），CC型134例（49．63％），T型等位基因为153例次（28．33％），C型等位基因为387例次（71．67％），2组间分布比较差异有统计学意义（ P ＜0．05）。高血压组α-内收蛋白Gly460Trp基因型分布情况为GlyGly型57例（21．76％），GlyTrp型为129例（49．23％），TrpTrp型为76例（29．01％），Gly型等位基因为243例次（46．37％），Trp型等位基因为281例次（53．63％）；健康对照组α-内收蛋白Gly460Trp基因型分布情况为 GlyGly 型63例（23．33％），GlyTrp型为124例（45．93％），TrpTrp型为83例（30．74％）， Gly 型等位基因为250例次（46．30％）， Trp 型等位基因为290例次（53．70％），2组间分布比较差异无统计学意义（ P ＞0．05）。结论中国人群中PPARγC161→T基因多态性与原发性高血压病相关；α-内收蛋白Gly460 Trp基因多态性与原发性高血压无相关性。%Objective To investigate the relationship between peroxisome proliferator activated receptor gamma (PPARγ) C161 →T,α-adducin gene Gly460Trp polymorphism and essential hypertension (EH).Methods Patients with essential hypertension (hypertension group) were 262 cases, and 270 normal persons (control group) were enrolled in the re-search, using polymerase chain reaction restriction fragment length polymorphism of loci PPARγgene and α-adducin gene Gly460Trp analysis, analyzed the 2 kinds of gene polymorphism frequency and compared between them .Results In hyperten-sion group, PPARγC161 → T genotype distribution as follows:10 cases of type TT (3.82%), type TC in 70 cases (26.72%), type CC in 182 cases (69.46%), the T allele was 90 cases (17.18%), C allele was 434 cases (82.82%);In healthy control group, distribution of PPARγC161→T gene type were TT type in 17 cases (6.30%), type TC in 119 ca-ses (44.07%), type CC in 134 cases (49.63%), the T allele was 153 cases (28.33%), C allele in 387 cases time (71.67%), compared the difference of distribution between the 2 groups showed statistically significant ( P <0.05).Hyper-tension group alpha-adducin Gly460Trp genotype distribution as follows:GlyGly type in 57 cases (21.76%), type GlyTrp in 129 cases (49.23%), type TrpTrp in 76 cases (29.01%), the Gly allele was 243 cases (46.37%), the Trp allele was 281 cases ( 53.63%); healthy controls 'α-adducin Gly460Trp genotype distribution as follows:GlyGly type in 63 cases (23.33%), type GlyTrp in 124 cases (45.93%), type TrpTrp in 83 cases (30.74%), the Gly allele was 250 cases (46.30%), the Trp allele was 290 cases (53.70%), no statistically significant difference of distribution between the 2 groups were found ( P >0.05).Conclusion Chinese population with PPARγC161→T gene polymorphism correlated with essential hypertension;no correlation between α-adducin gene Gly460Trp polymorphism and essential hypertension .