Objective To analyze the polymorphism of GH gene in Tibet miniature pig and Guangxi Bama miniature pig by PCR-RFLP. Methods The polymorphism of the -119bp- +486 bp fragment (growth hormone gene) of 108 Tibet miniature pigs and 132 Guangxi Bama miniature pigs was detected by PCR-Apal-RFLP and PCR-Hin6I-RFLP. Results (1) The PCR products were cut by Apal, and produced two alleles: A (449 bp + 101 bp + 55 bp) and B (316 bp + 133 bp + 101 bp + 55 bp). The frequency of allele A was higher than that of allele B. The frequency of the AA genotype was higher than that of the AB and BB genotypes. (2) The PCR products were cut by Hin6I, and produced four alleles: G1 (605 bp) , G2 (498 bp + 107 bp) , G3 (449 bp + 156 bp) and G4 (449 bp + 107 bp + 49 bp). The allele frequency of G4 was higher than that of Gl , G2 and G3. The frequency of allele G1s was the lowest. Both Tibet miniature pig and Guangxi Bama miniature pig exhibited moderate polymorphism. The frequencies of G2G3 , G2G4, G3G4 and G4G4 genotypes were higher than others. (3) There was no significant difference between the expressions of the allele in the Tibet miniature pig and Guangxi Bama miniature pig revealed by PCR-RFLPs. Conclusions The frequency of allele A of GH gene in Chinese miniature pig species, such as Tibet miniature pig and Guangxi Bama miniature pig, are rather high.%目的 对西藏小型猪和广西巴马小型猪生长激素基因(GH基因)部分序列的多态性进行分析.方法 采用内切酶ApaI和Hin6I对西藏小型猪(108头)和广西巴马小型猪(132头) GH 基因 - 119 bp～+486 bp 之间的区域进行PCR-RFLPs分析.结果 (1)从ApaI酶切产生的结果来看,ApaI酶切产生A(449 bp + 101 bp + 55 bp),B(316 bp + 133 bp + 101 bp + 55 bp)两种等位基因.等位基因A的频率高于等位基因B,等位基因A为优势基因.AA基因型频率高于AB和BB基因型频率;(2)从Hin6I 酶切产生的结果来看,Hin6I 酶切产生G1(605 bp)、G2(498 bp + 107 bp)、G3(449 bp + 156 bp)、G4(449 bp + 107 bp + 49 bp)四种等位基因.等位基因G4的频率高于等位基因G1、G2、G3.等位基因G1频率很低.基因型G2G3、G2G4、G3G4、G4G4的频率较高.(3)由酶切产生的基因和基因型多态性,发现西藏小型猪与广西巴马小型猪在该基因部分序列差异不显著(P>0.05).结论国内的优质实验用小型猪,如西藏小型猪和广西巴马小型猪等位基因A频率均较高.
展开▼
