摘要：
生长激素(growth hormone,GH)具有调节生长节奏的能力.本研究旨在探索GH、生长激素受体(growth hormone receptor,GHR)、胰岛素样生长因子1(insulin-like growth factor 1,IGF1)以及其受体IGFlR(insulin-like growth factor 1 receptor)在蒙古马(Equus caballus)不同器官中的表达差异.选取4匹平均年龄2岁的蒙古马为研究对象,利用qRT-PCR和免疫组化检测蒙古马不同器官中的GH、GHR、IGF1及IGF1R拘表达水平并进行分析.qRT-PCR结果表明,GH基因在脾脏中的表达高于淋巴、心脏、肝脏、肾脏、骨骼肌、尾肌(P＜0.05),GHR基因在肝脏表达量最高,其次为胰腺、尾肌、睾丸、心脏、骨骼肌、淋巴、肾脏、脾脏、肺脏(P＜0.05),IGF1基因在肝脏表达高于其他组织器官(P＜0.05),IGF1R基因的表达量依次为肺、肝脏、脾脏、睾丸、心脏、肾脏、淋巴、骨骼肌、尾肌、胰腺(P＜0.05).免疫组织化学检测结果显示,GH、IGF1蛋白在肝脏、肾脏、睾丸、骨骼肌和脾脏中均有分布,阳性反应强度不等.根据阳性细胞数得出,GH蛋白在肝脏、脾脏的阳性表达量高于其他组织器官(P＜0.05).IGF1的阳性表达量在肝脏中最高,脾脏、肾脏、睾丸次之,骨骼肌最少(P＜0.05).本实验通过RNA水平和蛋白水平两个层面的研究,发现GH、GHR、IGF1、IGF1R在蒙古马各器官中的表达具有差异性,为蒙古马的选育工作提供基础资料和科学依据.%Growth hormone (GH) has the ability to regulate the rhythm of growth.The present study was carried out to investigator the differential expression of GH,growth hormone receptor (GHR),insulin-like growth factor 1 (IGF1) and insulin-like growth factor 1 receptor (IGF1R) in different organs of Mongolia horse (Equus caballus).Four Mongolia horses with an average age of 2 years old were selected as the research object.The qRT-PCR and immunohistochemical detection were used to analyze the expression levels of GH,GHR,IGF1 and IGF1R in different organs of Mongolia horses.The results of RT-PCR results showed that the expression of GH in spleen were higher than in the lymph,heart,liver,kidney,skeletal muscle and tail muscle (P＜ 0.05).The expression of GHR in liver were higher than in the pancreas,tail muscle,testis,heart,skeletal muscle,lymph,kidney,spleen and lung (P＜ 0.05).The expression of IGF1 in liver were higher than in other organs (P＜0.05).The expression ofIGFIR in turn was lung,liver,spleen,testis,heart,kidney,lymph,skeletal muscle,tail muscle,pancreas (P＜0.05).Unequal immunoreaction intensity was observed in different tissues by immunohistochemistry.According to the number of positive cells,the positive expression of GH protein in liver and spleen was higher than in other organs (P＜0.05).It was found that the protein expression of IGF1 in liver was strongest,followed by the Spleen,kidney and testis,and that in skeletal muscle was weakest (P＜0.05).The present study showed that GH,GHR,IGF1,IGF1R had a differential expression in different tissues by studying at RNA level and protein level.This study provides a theoretical foundation and scientific basis for breeding of Mongolia horse.
