Objective To observe the morphology change of human umbilical cord mesenchymal stem cell (hUC-MSC) cultured on polypyrrole (PPy) under the conditions of different styles of electric stimuli. Method hUC-MSC were identified by flow cytometry and inverted phase contrast microscope. Passage 3 to 6 hUC-MSC were co-cultured with PPy. Current-controlled and voltage-controlled electric stimuli (ES) were applied to the cells. Cells cultured in aMEM with 10% fetal bovine serum and cells cultured on PPy without ES were used as control. Scan electron microscope (SEM) and inverted phase contrast microscope were used to observe the morphology change of hUC-MSC. Result The effect of ES on the adhesion and morphology change of hUC-MSC was evident and similar in different groups. Conclusion ES has distinct effect on hUC-MSC adhesion and morphology and can be used as a method of cell regulation. More research are needed.%目的 观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cell,hUC-MSC)与聚吡略共培养后,在不同通电条件下形态学变化.方法 体外分离培养hUC-MSC并进行鉴定.循环伏安法制备聚吡咯( polypyrrole,PPy)薄膜.选择第3～6代的hUC-MSC与pPy共培养,分别采用控制电压和控制电流的方法对细胞进行刺激,利用电镜及光镜观察细胞形态学变化.不通电组作为阴性对照.结果 电刺激对细胞贴附、形态有较大影响,不同形式的电刺激对细胞形态影响的比较类似.结论 电刺激结合生物导电材料对细胞的贴附和形态可造成显著改变,可作为调控细胞生长的手段之一,需要进一步研究其控制条件.
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