人结肠癌细胞系绿色荧光蛋白裸鼠移植瘤模型的建立与观察

摘要

Objective To establish ectopic colon tumor model in GFP nude, make a comparative study of the differences between tumor morphology, biological characteristics and pathology with ectopic colon tumor model in BALB/c nude.Methods Use KM12SM colon cancer cells of the logarithmic growth phase seeded in right axillary subcutaneous on the GFP nude and BALB/c nude.Observed time of tumor formation, tumor volume, pathological conditions and other. Results The average time of tumor formation in GFP nude was 4~5 d, BALB/c nude was 6~8 d;The first 20 days after inoculation, tumor volume of BALB/c nude group was about(3463.43 ±341.39)mm3, GFP nude was about(4923.46 ± 521.72)mm3.There are significant differences.Tumor cells growth activity, have relatively uniform size, some areas visible glandular formation on the microscope.Conclusion By comparison study, the GFP nude have certain advantages on the time of tumor formation, quality and other aspects in colon tumor model than the BALB/c nude.Provide more desirable animal model for investigating the mechanism of colon cancer lymph node metastasis, diagnosis and treatment.%目的：建立绿色荧光蛋白（ GFP）裸鼠结肠癌肿瘤模型，并与普通裸鼠结肠癌肿瘤模型进行比较，观察其在肿瘤形态学、生物学特性及病理学之间的差异。方法将处于对数生长期的KM12SM人结肠癌细胞分别接种于10只GFP裸鼠和10只裸鼠的右侧腋窝皮下，观察成瘤时间，肿瘤体积及病理形态等情况。结果 GFP裸鼠平均成瘤时间约为4～5 d，裸鼠约为6～8 d；接种第20天，裸鼠组测得体积约为（3463.43±341.39）mm3，GFP裸鼠组肿瘤体积约为（4923.46±521.72） mm3，存在显著差异。镜下见肿瘤细胞生长活跃，大小较为均匀，部分区域可见腺腔形成。结论经比较研究，GFP裸鼠在结肠癌肿瘤模型的成瘤时间，肿瘤质量等方面较裸鼠具有一定的优势，可为结肠癌转移的机制探讨和诊断治疗研究提供更为理想的动物模型。