绿色荧光蛋白标记的鼻咽癌放射抗拒性裸鼠移植瘤模型的建立及其活体成像观察

摘要

Objective To establish a GFP-labelled radioresistant xenograft tumor model of human nasopharyngeal carcinoma(NPC) and build the foundation for further study. Methods Lentivirus was used to establish a stable,radioresistant human NPC cell line expressing GFP(GFP-CNE-2R)and a corresponding non-radioresistant cell line(GFP-CNE-2).Both lines were subcutaneously implanted into BALB/c-nu nude mice;when the tumors had reached 1 cm in size,the animals were irradiated with 10 Gy in 1 fraction.Tumor proliferation and metastasis were analyzed in vivo using dynamic imaging technology. Results GFP-CNE-2R and GFP-CNE-2 lines were successfully established,and clone formation assays showed GFP-CNE-2R cells to be radioresistant.Xenograft tumors were grown from GFP-CNE-2R and GFP-CNE-2 cells,and growth of GFP-CNE-2R tumors was not significantly inhibited by radiation. Conclusion GFP-CNE-2R xenograft tumors are more radioresistant than GFP-CNE-2 xenograft tumors.%目的：建立绿色荧光蛋白（green fluorescent protein，GFP）标记的鼻咽癌放射抗拒性裸鼠移植瘤模型，为后续研究奠定基础。方法将以慢病毒转染的方法建立稳定表达GFP标记的人鼻咽低分化鳞癌细胞株GFP-CNE-2和放射抗拒性细胞株GFP-CNE-2R接种至裸鼠体内，建立皮下移植的肿瘤模型。待肿瘤直径约为1 cm时给予相同剂量10 Gy的X射线一次性照射，通过小动物活体成像仪观察移植瘤的生长和转移情况。结果成功建立了稳定表达GFP标记的放射抗拒性及放射敏感的人鼻咽低分化鳞癌细胞株GFP-CNE-2R和GFP-CNE-2。克隆形成实验显示GFP-CNE-2R细胞株具有放射抗拒性。GFP-CNE-2R和GFP-CNE-2细胞的皮下成瘤率均为100%，证实建模成功。相对于GFP-CNE-2裸鼠移植瘤， GFP-CNE-2R移植瘤的生长受照射抑制不明显。结论相对于GFP-CNE-2裸鼠移植瘤，GFP-CNE-2R裸鼠移植瘤具有更强的放射抗拒性。