不同来源高度免疫缺陷小鼠微卫星 DNA 遗传检测的分析

摘要

Objective To test and analyze the genetic background of highly immunodeficient mice from different sources.Methods Four highly immunodeficient mouse strains from different sources of NOD background were collected. 30 microsatellite DNA sites were detected, and the genotype can be displayed by gel electrophoresis and STR scanning. Results 17 microsatellite sites exhibit polymorphism in 20 mice of the four groups.There were 30 homozygous loci in the mice of groups A and B, and heterozygous in the other two groups.The genetic distance is minimum between groups A and B, showing a higher genetic similarity.Conclusions The genetic backgrounds are different in highly immunodeficient mice from different sources.%目的：对不同来源的高度免疫缺陷小鼠进行遗传背景的检测分析，旨在发现经遗传修饰的小鼠遗传背景是否符合近交系遗传特征。方法对收集到的4种不同来源以 NOD 为背景的高度免疫缺陷小鼠，选取30个微卫星 DNA 位点进行 PCR 检测，通过凝胶电泳结果和 STR 扫描确定基因型，进行遗传分析。结果4组20个样本中共有17个位点呈现多态性，A、B 两组小鼠30个微卫星位点表现为纯和，其他两组小鼠中不同位点均出现杂合个体；A、B 两组遗传距离最小，保持着较高的遗传相似度。结论研究表明，不同来源高度免疫缺陷小鼠遗传背景有差异。