CKLF1-C19对昆明小鼠的急性毒性研究

摘要

Objective To explore the acute toxicity effect of CKLF1-C19,a polypeptide of chemokine-like factor 1(CKLF1),on the KM mice. Methods A total of 40 KM mice,half male and half female,were randomly divided into 2 groups. The mice in the experimental group were injected with CKLF1-C19 at a dose of 25 mL/kg(100 μg/mL,1 mg/mL and 10 mg/mL)through the tail vein,and those in the control group received an equal volume of sterile saline solution. Changes in the body weight of the mice were recorded the day after treatment, and the general conditions of mice in the experimental group were observed closely and compared with the normal group. Then blood samples were taken from the abdominal aorta to measure liver and kidney function. Tissue samples of liver, kidney, spleen and lung were taken for histopathological examination by HE staining. Results In the maximum tolerance test,the mice of the two groups were in good condition, and their body weight was increased gradually, without significant difference between the experimental group and the control group(P > 0.05). There was no death within 14 days. The blood biochemical indexes of liver and kidney function showed no significant differences between the two groups(P > 0.05). The gross appearances of heart, liver,kidney,spleen and lung were normal in the two mouse groups, and the pathological examination with HE staining showed normal clear structure with no obvious changes in these organs of each group. Conclusions Our results demonstrate that CKLF1-C19 has no acute toxicity effect on mice.%目的 研究趋化素样因子1(CKLF1)的多肽CKLF1-C19对昆明小鼠的急性毒性作用.方法 以25 mL/kg的CKLF1-C19(100 μg/mL、1 mg/mL、10 mg/mL三种浓度)剂量注射实验组小鼠,对照组则给予同等体积生理盐水,给药后隔天记录小鼠的体重变化,密切观察实验组小鼠的一般状况.结束实验后留取小鼠腹主动脉血检测肝肾功能;留取肝、肾、脾、肺组织观察外观、做HE染色及常规组织病理学检查.结果 两组小鼠一般状况良好,体重缓慢增长,两组小鼠体重变化差异无显著性(P> 0.05),14 d后均无死亡情况;两组小鼠肝肾功能相关的血液生化结果差异无显著性(P> 0.05);肉眼观察两组小鼠心脏、肝、肾、脾、肺等脏器正常,肝、肾、脾、肺组织HE染色后结构清晰,未见异常.结论 CKLF1-C19对小鼠无急性毒性作用.