EB病毒相关性噬血细胞综合征患儿T淋巴细胞CD69的表达及意义

摘要

目的：探讨外周血T淋巴细胞CD69表达在EB病毒相关性噬血细胞综合征（epstein-barr virus-associated hemophagocytic syndrome， EBV-AHS）患儿中的变化及意义。方法应用流式细胞术检测EBV-AHS患儿（急性期28例，缓解期21例）及30例健康对照儿童外周血CD3＋CD4＋CD69＋及CD3＋CD8＋CD69＋的表达水平，对检测结果进行统计学分析。结果三组受试者间CD3＋CD4＋CD69＋及CD3＋CD8＋CD69＋的表达水平差异均有统计学意义（P均＜0．01）；急性期组CD3＋CD4＋CD69＋及CD3＋CD8＋CD69＋的表达水平明显高于缓解期组及对照组，且差异均有统计学意义（P均＜0．01）；缓解期组CD3＋CD4＋CD69＋及CD3＋CD8＋CD69＋的表达水平明显高于对照组，且差异均有统计学意义（P均＜0．01）。结论 T淋巴细胞的过度活化在EBV-AHS发病过程中起重要作用，是EBV-AHS重要的发病机理之一。检测T淋巴细胞CD69的表达有利于临床判断治疗效果，并为应用免疫调节剂提供理论依据。%Objective To explore the change and significance of the peripheral blood T lymphocyte CD69 expression in children patients with epstein-barr virus-associated hemophagocytic syndrome (EBV-AHS). Methods The expression levels of CD3+CD4+CD69+and CD3+CD8+CD69+in EBV-AHS children(28 cases in acute phase and 21 cases in paracmasis) and 30 cases of healthy controls were detected by flow cy-tometry method. The results were analyzed statistically. Results There were statistical significance in the dif-ferences of CD3+CD4+CD69+and CD3+CD8+CD69+expression levels among three groups(Pall<0.01). The ex-pression levels of CD3+CD4+CD69+and CD3+CD8+CD69+in acute phase group were all higher than that of paracmasis group and control group, and the differences all had statistiacal significance (Pall<0.01). The ex-pression levels of CD3+CD4+CD69+and CD3+CD8+CD69+in paracmasis group were all higher than that of con-trol group, and the differences all had statistiacal significance (Pall<0.01). Conclusion Excessively activate of T lymphocyte play an important role in the EBV-AHS attack procession. Detection of T lymphocyte CD69 expression is helpful for treatment effect judgment in clinical , and provide theoretical basis for application of immune modulators.