RKIP PTEN基因启动子区甲基化与肺癌的关系

摘要

目的:探讨RKIP基因和PTEN基因启动子区甲基化与肺癌及其临床病理特征的关系.方法:采用甲基化特异性PCR(MSP)法,分析肺癌组织及相应癌旁正常肺组织中RKIP和PTEN启动子区甲基化表达情况.结果:45.8%(38/83)的肺癌组织和13.3%(11/83)的癌旁肺组织RKIP基因启动子区发生甲基化,51.8%(43/83)的肺癌组织和15.7%(13/83)的癌旁肺组织PTEN基因启动子区发生甲基化,癌组织中RKIP和PTEN启动子区甲基化率显著增高(P<0.05);发生淋巴结转移的43例肺癌组织中,27例RKIP基因启动子区发生甲基化,30例PTEN基因启动子区发生甲基化,淋巴结转移组RKIP及PTEN基因启动子区甲基化均显著高于无淋巴结转移组(P<0.05).结论:肺癌中RKIP和PTEN基因启动子区甲基化,可能是肺癌发生、发展及转移的重要原因之一.%Objective: To discuss the relationship between the promoter methylation pattems of RKIP and PTEN in lung cancer and their clinicopathologic features.Methods: Methylation-specific polymerase chain reaction PCR ( MSP-PCR ) was employed to detect the promoter methylation of RKIP and PTEN in lung cancer tissues and as well as their paraneoplastic tissues in 83 lung cancer patients.Results: Promoter methylation of RKIP was found in 45.8％ ( 38/83 ) of the total lung cancer tissue samples and 13.3％ ( 11/83 )of the paraneoplastic lung tissue samples.The differences were significant between the two groups ( P ＜ 0.05 ).There was promoter methylation of PTEN in 51.8％ ( 43/83 ) of the lung cancer tissue samples and 15.7％ ( 13/83 ) of the paraneoplastic lung tissue samples.Of the 43 cases with lymph node metastasis, RKIP promoter methylation was observed in 27, and PTEN promoter methylation was found in 30.The methylation rate in the promoter region was significantly higher in cases with nodal metastasis than in cases without nodal metastasis ( P ＜ 0.05 ).Conclusion: Promoter methylation pattems of RKIP and PTEN in lung cancer may be considered as important causes of the carcinogenesis, progression, and metastasis of lung cancer.