目的:通过β受体阻滞剂普萘洛尔作用于小鼠EOMA血管瘤细胞体外增殖及凋亡的实验研究,初步探讨普萘洛尔治疗血管瘤的机制.方法:体外培养EOMA细胞,使用不同浓度普萘洛尔分别作用于EOMA细胞24、36、48 h,应用噻唑蓝(MTT)法检测细胞存活率、吖啶橙染色检测细胞凋亡情况,观察普萘洛尔对EOMA细胞体外增殖和凋亡的影响.结果:作用24 h后,随剂量增加,EOMA细胞存活率逐渐下降,与对照组相比,至药物浓度为75 μmol/L时有显著差异(P<0.05),继续增加至800 μmol/L时,细胞存活率接近0,同时吖啶橙染色示凋亡细胞逐渐增多,与对照组相比,至药物浓度75 μmol/L时,细胞凋亡率有显著差异(P <0.05).36 h组和48 h组变化趋势与24h组相似.结论:普萘洛尔在体外可有效抑制小鼠EOMA血管瘤细胞的增殖并促进其凋亡,这一作用呈现明显的剂量-时间效应依赖性.%Objective: To explore the primary mechanism of propranolol treatment on hemangioma. Methods: Mouse hemangio-endothelioma endothelial (EOMA) cells cultured in vitro were used as the cell models in our study. Cells were treated using propranolol at different concentrations ( 5 umol/L to 800 umol/L ) for 24 h to 48 h. Cell proliferation was analyzed by MTT assay, and apoptosis was studied by acridine orange (AO) staining. Results: After 24 h to 48 h treatment, significant differences of cell viability and apoptosis were noted ( P < 0.05 ) at the concentration of 75 μmol/L compared with the control group ( 0 μmol/L ). When the dose was increased, cell viability dropped, whereas apoptosis dramatically increased. Conclusion: Propranolol can effectively inhibit the proliferation and induce the EOMA cell apoptosis in vitro.
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