HP感染与胃癌及癌前病变组织中hTERT表达的关系

摘要

Purpose To investigate the infection of helicobacter pylori( HP )and expression of hTERT protein in gastric cancer and precancerous lesions. Methods HP infection was detected by Warthin-Starry method, the expression of hTERT protein were examined by immunohistochemical technique SP method. Results HP infection in intestinal metaplasia, epithelial dysplasia of stomach and gastric carcinomas were 60. 61％ ,66. 67％ and 58. 00％ ,which were significantly higher than those in normal gastritis mucosa( P ＜ 0. 05 ). The positive expression rate of hTERT protein were 68. 18％ ,72. 22％ and 78. 00％ , which were significantly higher than those in normal gastritis mucosa( P ＜ 0. 01 ). The positive expression rate of hTERT protein in 93 cases of gastric mucosa lesion of positive HP infection ( 88. 17％ ) was significantly higher than that in 59 negative cases ( 47. 46％ ,P ＜ 0. 01 ). Conclusions HP infection and hTERT protein expression are associated with gastric carcinomas; that HP infection can result in decreased expression of hTERT protein may be one of molecular mechanism by which HP infection leads to gastric carcinoma.%目的 探讨胃癌及癌前病变中幽门螺杆菌(helicobacter pylori,HP)感染情况及与hTERT蛋白表达的相互关系.方法 采用Warthin-Starry银染法、免疫组织化学SP法分别检测66例胃黏膜肠化生、36例异型增生、50例胃癌及10例正常胃黏膜HP感染及hTERT蛋白表达情况.结果 胃黏膜肠化生、异型增生、胃癌中HP感染率分别为60.61%、66.67%、58.00%,均显著高于正常胃黏膜,差异有显著性(P＜0.05).胃黏膜肠化生、异型增生及胃癌中hTERT蛋白阳性表达率分别为68.18%、72.22%、78.00%,均显著高于正常胃黏膜(P＜0.01).93例HP感染阳性组hTERT蛋白表达阳性率为88.17%,59例HP感染阴性组hTERT蛋白表达阳性率为47.46%,两组比较差异有显著性(P＜0.01).结论 HP感染、hTERT蛋白与及胃癌的发生有关,HP引起hTERT蛋白表达水平的上升可能是HP感染致胃癌的另一分子机制.