胃癌中HER-2、MT1-MMP及CD147蛋白的表达及意义

摘要

目的 观察HER-2、MT1-MMP及CD147蛋白在胃癌中的表达和相关性及其与胃癌生物学行为的关系.方法 应用免疫组化SABC法检测10例正常胃组织和178例胃癌组织中HER-2、MT1-MMP和CD147蛋白的表达.结果 HER-2、MT1-MMP和CD147蛋白在正常胃组织和胃癌组织的表达差异均有显著性(P<0.05);且它们在分化程度低、侵及浆膜层以及有淋巴结转移的胃癌中的表达均明显高于分化程度高、未侵及浆膜层以及无淋巴结转移胃癌组(P<0.01).在胃食管连接部的HER-2蛋白表达高于胃部胃癌,HER-2与MT1-MMP蛋白在胃癌中表达有相关性,但差异差异无显著性,Pearson相关系数为0.301(P=0.05).CD147与MT1-MMP蛋白在胃癌中的表达亦有相关性,Pearson相关系数为0.887(P<0.01).结论 HER-2、MT1-MMP及CD147蛋白在胃癌中均高表达,HER-2蛋白、CD147蛋白的表达均与MT1-MMP的表达相关,三者可能共同参与胃癌的侵袭和转移.%Purpose To investigate the expression of HER-2, MT1-MMP and CD147 in gastric carcinoma and to find out their correlations with the biologic behaviors of the tumor. Methods HER-2, MT1-MMP and CD147 proteins were detected by immunohistochemi-cal staining in 10 cases of normal gastric tissue and 178 cases of gastric carcinoma. Results All of the expression level of HER-2, MT1-MMP and CD147 protein in gastric carcinoma tissues was significantly higher than that in normal gastric tissues ( P <0. 05 ). The expression of them in the subgroup of poorly-differentiated with serosal invasion and lymph nodes metastasis was significantly higher than the other subgroup of well-differentiated without serosal invasion and lymph nodes metastasis ( P <0. 01 ). And the expression of HER-2 protein in the esophagogastric junction was higher than in the stomach. There was a positive correlation trend between the expression of HER-2 and MT1-MMP protein in gastric carcinoma, but without significant difference, with Pearson coefficient of 0. 301 ( P = 0.05 ). The positive correlation was found between CD147 and MT1-MMP protein in gastric carcinoma, with Pearson coefficient 0. 887 ( P <0. 01 ). Conclusions Over-expression of HER-2, MT1-MMP and CD147 is detected in gastric carcinoma, with close relationships of HER-2 and CD147 with MT1-MMP, which suggests that all the three proteins may be involved in the invasiveness and metastasis of gastric carcinoma.