53BP1在宫颈鳞状细胞癌及其癌前病变中的表达

摘要

Purpose To investigate the expression of 53BP1 in squamous cell carcinoma and precancerous lesions of the cervix, and to expolore the role of 53BP1 in the carcinogenesis of cervical cancer. Methods Immunohistochemical En Vision was used to detect the expression of 53BP1 in 10 cases of normal cervix tissue, 20 cases of cervical intraepithelial neoplasm l( CIN1 ), 20 cases of CIN2, 20 cases of CIN3, and 20 cases of squamous cell carcinoma. Results Focal positive expression of 53BP1 was seen in 2 cases of normal cervical tissue and the positive rate of 53BP1 in the normal cervix was 20%. The positive rates of 53BP1 in CIN1, CIN2, CIN3 and cancer were all 100% . There was significant differences between normal tissue and CIN1, CIN2, CIN3 and cancer( P < 0. 01 ). The diffuse positive rates of 53BP1 in the normal cervix and CIN1 were 0, but the diffuse positive rates of 53BP1 in the CIN2, CIN3 and cancer were 55% , 70% and 100% . The diffuse positive rates of 53BP1 in the CIN2, CIN3 and cancer were higher than that in normal tissue and CIN1 ( P <0. 01 ). There was no difference of'53BPl expression between CIN2 and CIN3 ( P = 0. 327 ). The diffuse positive expression of 53BP1 in the squamous cell carcinoma was higher than that in CIN3 and CIN2 ( P =0. 020, P =0. 001 ). Conclusions Expression of 53BP1 may be a marker for genomic instability and genomic instability may be a early event in the carcinogenesis of cervical cancer.%目的 探讨53BP1在宫颈鳞状细胞癌及其癌前病变中的表达,阐明53BP1在宫颈鳞状细胞癌发生中的作用.方法 应用免疫组化EnVision法检测10例正常宫颈组织、20例宫颈鳞状上皮内瘤变1级(cervical intraepithelial neoplasm 1,CIN1)、20例CIN2、20例CIN3和20例宫颈鳞状细胞癌中53BP1的表达.结果正常宫颈组织中仅有2例53BP1呈局灶性表达,阳性率为20%,而53BP1蛋白在CIN1～3和宫颈癌组织中阳性率均为100%,差异有统计学意义(P<0.01).53BP1在正常宫颈组织和CIN1中弥漫阳性率均为0,而在CIN2、CIN3和宫颈癌组织中弥漫阳性率分别为55%、70%和100%,53BP1在CIN2、CIN3和宫颈癌组织中的弥漫阳性表达明显高于正常宫颈组织和CIN1(P<0.01).53BP1蛋白弥漫阳性表达在CIN2与CIN3之间差异无显著性(P=0.327).宫颈癌中53BP1蛋白弥漫阳性表达明显高于CIN3和CIN2,差异有统计学意义(P=0.020;P=0.001).结论 53BP1表达水平可作为基因组不稳定性的标记物,基因组不稳定性在宫颈癌发生过程中可能是一早期事件.