目的 探讨结直肠癌组织中miR-21的表达及其临床病理学意义.方法 采用TaqMan实时定量RT-PCR法检测43例结直肠癌组织及其癌旁正常组织中miR-21的表达,分析miR-21的表达与结直肠癌临床病理因素之间的关系.结果 miR-21在结直肠癌组织中的表达明显高于癌旁正常组织(P0.05).结论 miR-21在结直肠癌中表达升高,miR-21高表达同结直肠癌病理学分级、淋巴结转移及术后生存率密切相关,miR-21可能在大肠癌的发生、发展过程中发挥重要作用.%Purpose To investigate the miR-21 expression in cancerous tissues from patients with colorectal carcinoma ( CRC ) and to evaluate its dinicopathologic significance. Methods TaqMan real-time quantitative RT- PCR was used to detect the miR-21 expression in cancerous tissues and normal tissues from 43 patients with colorectal carcinoma and to observe its relationship with dinicopathologic parameters. Results There was a significantly higher expression of miR-21 in CRC tissues in comparison with adjacent normal colon tissues ( P <0. 05 ). High miR-21 expression was associated with dinicopathologic features, such as tumor grade, lymph node metastasis and patient survival ( P <0. 05 ). However, there was no relationship between miR-21 expression and other clinicopathologi-cal parameters including sex, age and tumor site. Conclusion The expression of miR-21 was up-regulated in colorectal carcinoma. High miR-21 expression correlates with tumor grade, lymph node metastasis and patient survival, which suggests that mir-21 may play an important role in the development and progression of colorectal carcinoma.
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