COX-2和VEGF在结直肠癌组织中的表达及其临床病理学意义

摘要

Objective:The study test the expression of cyclooxygenase-2 ( COX-2 ) and vascular endothelial growth factor( VEGF) in colorectal cancer tissues and normal colon tissues,and detect the correlation of colorectal cancer cases in the clinic pathological parameters, to further understand the clinic pathological sense of the COX-2 and VEGF in the invasion and metastasis of colorectal cancer. Methods:117 paraffin specimens in the case of colorectal cancer after operation and 15 in the cases of normal colorectal mucosa resection were collected. Immunohistochemical method was used to detect the level of expression of COX-2 and VEGF, and analysis the difference of expression of COX-2 and VEGF in colorectal cancer tissue and normal colorectal mucosa tissues. Results:the positive expression of COX-2 and VEGF in colorectal cancer tissues was significantly higher than that in normal colorectalmucosa, the difference was statistically significant ( P<0. 05 ). There was statistically significant difference for the expression of COX-2 and VEGF in colorectal cancer tissues with tumor cell invasion, tumor differentiation,lymph node metastasis,TNM stage,(P<0. 05). While no significant differences with gender,age,tumor location,tumor diameter,liver metastasis,and survival status(P>0. 05). The expression of COX-2 and VEGF had significant positive correlation. Conclusion:the expression of COX-2,VEGF in colorectal cancer tissues was higher than that in normal colorectal tissues,and the expression of COX-2 and VEGF in tumor tissues have a positive correlation with synergistic effect, which prove that COX-2,VEGF plays a role in tumor occurrence,development,and they can be used for judging tumor occurrence,and can be useful for the prognosis,diagnosis and treatment of tumor.%目的：：本课题通过检测COX-2与VEGF在结直肠癌组织和正常结肠组织中的表达情况，探讨它们与结直肠癌病例的各项临床病理参数的关系及相关性，了解COX-2和VEGF在结直肠癌浸润和转移中的作用。方法：本文收集了结直肠癌根治术后病理存档的石蜡标本117例及正常结直肠黏膜组织15例。应用免疫组织化学方法检测COX-2和VEGF表达水平并分析其在结直肠癌组织中的表达及其临床病理学意义。结果：COX-2及VEGF在结直肠癌组织中的阳性表达明显高于正常结直肠黏膜组织，两者差异具有统计学意义(P<0.05)。COX-2及VEGF在结直肠癌组织中的表达与肿瘤浸润深度、分化程度、淋巴结转移情况、TNM分期间差异均有统计学意义(均P<0.05)。 COX-2与VEGF在结直肠癌中的表达有显著正相关性。结论：COX-2、VEGF在结直肠癌组织中的表达明显高于正常结直肠组织，且肿瘤中的表达有着正相关性有着协同效应，表明COX-2、VEGF通过一定的机制相互作用，共同参与结直肠癌的发生发展过程，这可能对结直肠癌的诊治有一定的帮助。