目的：探讨硫氧还蛋白还原酶-1(thioredoxin reductase-1, TrxR1)在非小细胞肺癌(non-small cell lung cancer, NSCLC)中的表达,及其在干预肺癌细胞中所产生的效应。方法应用免疫组化法检测118例NSCLC和27例正常肺组织中TrxR1的表达,并分析其与NSCLC临床病理参数的关系；应用半定量RT-PCR法检测13例NSCLC和4例正常肺组织中TrxR1 mRNA的水平。 Western blot法检测正常肺上皮细胞和不同肺癌细胞中TrxR1蛋白的水平；半定量RT-PCR法检测正常肺上皮细胞和不同肺癌细胞中TrxR1 mRNA的水平。沉默A549细胞中TrxR1的表达,并比较沉默前后细胞的增殖情况。结果 NSCLC的免疫组化及半定量RT-PCR结果显示TrxR1在癌细胞中过表达( P<0．05)；使用shRNA沉默肺癌细胞中TrxR1的表达后,细胞增殖明显降低。结论 TrxR1 mRNA和蛋白在NSCLC中过表达,提示TrxR1有可能是NSCLC的靶标分子。%Purpose To explore the expression of thioredoxin reductase-1 ( TrxR1 ) in non-small cell lung cancer and to observe the effects of TrxR1 on non-small cell lung cancer ( NSCLC) . Methods Immunohistochemistry was used to determine the expression of TrxR1 in 118 NSCLC tissues and 27 normal lung tissues, and to analyze its relationships with clinical pathological features. TrxR1 mR-NA levels were measured by quantitative RT-PCR in 13 cases of NSCLC and 4 cases of normal lung tissue. TrxR1 protein and its mR-NA levels were assessed by Western blotting and quantitative RT-PCR in normal lung epithelial cells and different lung cancer cell lines. After the silence TrxR1 expression in A549 cells, the changes of proliferation were compared. Results The data of immunohis-tochemistry and quantitative RT-PCR showed the overexpression of TrxR1 in NSCLC ( P <0. 05 ) . After using shRNA silencing of TrxR1 in lung cancer cells, cell proliferation changed significantly. Conclusion There are a high mRNA and protein levels of TrxR1 in NSCLC. TrxR1 may be a critical therapeutic target for the treatment in NSCLC.
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