联合免疫荧光和荧光原位杂交法检测TNFAIP3基因在结外鼻型NK/T细胞淋巴瘤中的异常及意义

摘要

目的 观察TNFAIP3基因在结外鼻型NK/T细胞淋巴瘤中的异常,并评估其预后价值.方法 利用联合免疫荧光和荧光原位杂交(fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasm,FICTION)法检测109例结外鼻型NK/T细胞淋巴瘤中TNFAIP3基因的表达.结果 在检测成功的78例结外鼻型NK/T细胞淋巴瘤中发现32.1％ (25/78)病例存在TNFAIP3基因缺失.TNFAIP3基因缺失与患者性别、年龄、B症状、肿瘤分期、乳酸脱氢酶水平无明显相关性(P均＞0.05),与国际预后指数(international prognostic index,IPI)有关(P=0.019).结论 结外鼻型NK/T细胞淋巴瘤中存在的高比例TNFAIP3缺失,且与IPI关联,提示TNFAIP3缺失具有重要的预后价值.%Purpose To explore the prognostic value of deletion of the TNFAIP3 gene in natural killer/T-cell lymphoma,nasal type detected with fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasm (FICTION).Methods FICTION was performed to detect the abnormalities of TNFAIP3 gene in 109 cases of natural killer/T-cell lymphoma,nasal type.Results TNFAIP3 deletion was found in 25/79 detectable cases.The deletion of TNFAIP3 was positively correlated with the International Prognosis Index (IPI) (P =0.019).Conclusion Frequent deletion of TNFAIP3 was associated with IPI in natural killer/T-cell lymphoma,nasal type,suggesting the important prognostic value of TNFAIP3 in the natural killer/T-cell lymphoma,nasal type.