流动相离子色谱法同时测定植物中残留的矮壮素和缩节胺

摘要

以离子对试剂作流动相,采用离子对抑制电导检测法同时测定植物中残留的矮壮素和缩节胺.简单处理后的样品经过Dionex IonPac NG1保护柱和NS1分离柱,在流速为1.00 mL/min、淋洗液为1.00 mmol/L九氟戊酸(作为离子对试剂)-7%(体积分数,下同)乙腈时等度洗脱分离,能够快速稳定出峰,且被测物与其他干扰离子充分分离.矮壮素和缩节胺的检出限分别为0.154 6和0.171 4 mg/L,在1～100 mg/L范围内具有良好的线性关系和重现性.对实际样品进行测定,矮壮素和缩节胺的回收率范围分别为96.06%～104.6%和98.53%～103.7%,相对标准偏差小于3%.该方法分析结果令人满意,可以满足矮壮素和缩节胺常规的定性、定量分析需求.%Ion-pair reagent was used as mobile phase for the simultaneous separation of chlormequat chloride and mepiquat chloride in plants.The rapid separation was performed on a Dionex IonPac NG1 guard column and a Dionex IonPac NS1 analytical column using 1.0 mL/min of the eluent mixture of 1.00 mmol/L nonafluoropentanoic acid ( as ion-pair reagent) and 7％ acetonitrile, and the detection was achieved by a suppressed conductivity detector.The method provided good resolution of the analyte peaks without any interference.The detection limits of chlormequat chloride and mepiquat chloride were 0.154 6 mg/L and 0.1714 mg/L, respectively.The linear calibration curves were obtained in the range of 1 - 100 mg/L.The mean recoveries in the ranges of 96.06％ - 104.6％ for chlormequat chloride and 98.53％103.7％ for mepiquat chloride were obtained in real samples.The method requires only simple sample preparation and the technique is suitable for routine quality control analysis.